微管吸吮法的改进及用于测定细胞在材料表面的黏附力  

Modification and Application of the Micropipette Aspiration Method for Measuring the Cell Adhesion Force on Material Surface

作　　者：高珍[1,2] 王松[1,2] 朱鹤孙[1] 许家超[3] 

机构地区：[1]北京理工大学材料科学研究中心,北京100081 [2]北京理工大学材料科学与工程学院,北京100081 [3]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《生物医学工程学杂志》2009年第3期508-511,共4页Journal of Biomedical Engineering

基　　金：国家"九七三"计划项目资助(2005CB623906)

摘　　要：通过选取"具有相对稳定黏附力的圆细胞",对微管吸吮法进行了改进。以丝素蛋白膜和经褐藻糖胶改性的丝素蛋白膜作为基质,测定了不同培养条件下人脐静脉内皮细胞在基质表面的初始黏附力。结果表明,改进后的微管吸吮法可使测定速度得到较大提高;改变培养温度和培养时间,所测得不同材料表面的黏附力趋势均相同,测定结果具有较好的重复性;可区分两种不同产地褐藻糖胶的少量引入对丝素蛋白膜材料表面的内皮细胞黏附力的不同作用,具有较高的敏感度。The micropipette aspiration technique was improved by selecting the uniform cells in round shape （UCR） from a number of adhered ceils on the testing surface so as to measure the adhesion forces during the initial adhesion stage. Silk fibroin film and silk fibroin/fucoidan film were used as testing substrates. The testing results revealed that the modified micropipette method was more rapid for testing. The tendency of cell adhesion force on different materials surface under different conditions remained unchanged. The testing results showed good reproducibility. It was easy to distinguish small differences in the adhesiveness of endothelial cell on the silk fibroin based materials, thus exhibiting the high sensitivity of the improved method.

关 键 词：细胞黏附力 微吸管 丝素基材料 

分 类 号：R318.08[医药卫生—生物医学工程]