培养条件对三角帆蚌外套膜离体上皮组织珍珠质分泌能力的影响(简报)  

EFFECT OF INCUBATION CONDITIONS ON THE NACRE SECRETION CAPACITY OF IN VITRO EPITHELIUM FROM MANTLE OF HYRIOPSIS CUMINGⅡ

作　　者：陈永富[1] 钱国英[1] 

机构地区：[1]浙江万里学院生物与环境学院,宁波315100

出　　处：《分子细胞生物学报》2009年第3期249-255,共7页Journal of Molecular Cell Biology

基　　金：浙江省农业科技重大攻关项目(2006C22030)

摘　　要：珍珠是由珍珠贝外套膜的上皮细胞受到外源刺激物刺激形成珍珠囊（pearl—sac），并由珍珠囊产生的钙质分泌物．其分泌物逐渐包围刺激原．使之体积急剧增长而形成的，珍珠质（nacre）是由大于95％的碳酸钙晶体与约5％的角壳蛋白组成的生物矿化产物。因此珍珠贝的外套膜在珍珠形成中起着重要的作用。珍珠贝外套膜的体外培养已开展了一些初步的研究工作。The effects of different calcium ion concentration in culture medium of epithelim, varient pH, and nucleus rotundus present or absent on the calcium metabolism of epithelim from mantle of Hyriopsis cumingii work were investigated in this study. With the variance of calcium ion concentration and pH, the results obtained by using polarizing microscope showed that there was no significant difference on calcium ion containing of epithelim cells between nucleus rotundus present and absent. Meanwhile, the reproduction of epithlim cell in mantle was occurred after incubation and it was further transferred to form pearl sac. The complete pearl sac is formed when connective tissue cells around epithlim cell of pearl sac. An obvious birefringence was observed by using po- larizing microscope and its strength was positively affected by the incubation duration and calcium ion concentration of culture medium. The results demonstrated that with the development of cell and tissue of mantle, the absorption of calcium carbonate from culture medium was occurred and there existed the significant conelation between surrounding calcium ion concentration and absorption or secretion of cultures. The largest quantity of calcium sphere was occurred when the calcium ion concentration was 200ml/L, which was very significantly higher than that of others （p〈0.01）. It was also found that the calcium ion absorption was affected by the euhure tissue and the status of culture. Higher calcium ion requirement was in primary culture than that in subculture. The calcium ion absorption and secretion was higher in tissue gobbet than that in single cell. A high significant correlation was observed between calcium ion absorption （Y） and pH （X）： Y=52.34-5601.23 X （r=0.9661, p〈0.05）. The calcium ion concentration 200mg/L and pH 7.2-7.5 are suggested for incubation conditions of epithelim primary culture in mantle of Hyriopsis cumingii.

关 键 词：外套膜 组织培养 细胞培养 钙离子浓度 双折射现象 

分 类 号：S917.4[农业科学—水产科学]