贵州白香猪母系与父系遗传检测  

Male and Female Genetic Identification on Guizhou White Xiang Pig

作　　者：王兆龙[1,2] 刘若余[1,2] 田甜[1,2] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州大学贵州省动物遗传育种与繁殖重点实验室,贵阳550025

出　　处：《山地农业生物学报》2009年第3期240-244,共5页Journal of Mountain Agriculture and Biology

基　　金：2007年贵州省农业厅地方畜禽保护项目资助

摘　　要：采用PCR测序技术分析贵州白香猪两品系(Ⅰ系、Ⅱ系)mtDNA D-loop区和Y染色体SRY基因编码区序列。结果表明,在遵循母系遗传的mtDNA D-loop区,贵州白香猪Ⅰ系具有3种单倍型H1、H2和H3;Ⅱ系具有1种单倍型H1;在遵循父系遗传的SRY基因编码区,两品系共享单倍型GWXPⅠ。说明贵州白香猪Ⅰ系具有3个母系血统,Ⅱ系具有1个母系血统;Ⅰ系和Ⅱ系具有1个父系血统。通过与GenBank上收录的其他猪品种的同源序列比对发现,贵州白香猪具有独特的线粒体遗传结构,且其线粒体基因纯合性较高。Mitochondrial DNA D - loop and the coding region sequence of Y chromosome SRY gene in two lines of Guizhou White Xiang Pig（ GWXP line Ⅰ and line Ⅱ ）were analyzed by PCR sequencing technique in the present work. In maternal inheritance mtDNA D -loop region, GWXP line Ⅰ demonstrated three Mitochondrial haplotypes, which were H1, H2 and H3. However, line Ⅱ had only one haplotye, which was HI. To the coding region of SRY gene in paternal inheritance, the two lines shared haplotype GWXP Ⅰ . Therefore, it indicated that there were three matemals blood hne- ages in line Ⅰ and one maternal blood lineage in line Ⅱ. Furthermore, there was only one paternal blood lineage in both line I and line Ⅱ. Compared with the homologous sequences available from GenBank, it can be concluded that GWXP had special haplotypes of mtDNA. Additionally, its Mitochondrial gene was more homozygous.

关 键 词：贵州白香猪 遗传检测 SRY基因 MTDNA D—loop 

分 类 号：S828.892[农业科学—畜牧学]