土荆皮酸诱导卵巢癌细胞系SKOV3凋亡的实验研究  

Experimental research on apoptosis of ovarian cancer cell line SKOV3 induced by pseudolaric acid B

作　　者：李克深[1] 胡云[2] 霍贵成[1] 吴效科[2] 侯丽辉[3] 翟玲[3] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]解放军第211医院妇产科,哈尔滨150080 [3]哈尔滨医科大学附属第一医院,哈尔滨150001

出　　处：《中华中医药杂志》2009年第7期921-923,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江省自然科学基金资助项目(No.AD200647);黑龙江省博士后基金资助项目(No.LBH-Z06159)

摘　　要：目的:研究土荆皮酸(PAB)对体外培养的SKOV3细胞的生物学效应和作用机制。方法:用MTT法、流式细胞术、电镜检测体外培养的SKOV3细胞在PAB作用下的增殖抑制及凋亡程度,用RT-PCR法检测P53/Bcl-2/Bax的表达水平。结果:①PAB对SKOV3细胞的增殖抑制作用随浓度升高而明显增强,其IC50约10μmol/L。②流式细胞术证实PAB呈浓度依赖性改变细胞周期分布,一方面降低G0/G1期的细胞比例,另一方面增高G2/M期细胞的比例。③RT-PCR法显示SKOV3细胞在10μmol/LPAB作用12、24、48h均显示Bax上调和Bcl-2的下调,P53则未检测到表达。结论:在体外PAB能抑制SKOV3细胞增殖,诱导其凋亡,其诱导凋亡机制可能与Bax的上调和Bc1-2的下调相关。Objective： To study the effect of pseudolaric acid B （PAB） on ovarian cancer cell line SKOV3 and its mechanism. Methods： Growth inhibition/apoptosis and cell cycles changes of SKOV3 cell line induced by PAB measured by means of MTT/flow cytometry/electronic microscope. RT-PCR technique was used to study the transcriptional changes of gene P53/Bax/Bcl-2. Results： The growth inhibition of SKOV3 cell line induced by PAB was enhanced in time-dependent and dosedependent pattern and IC50 of 72 hours was 10μmol/L; Apoptosis was detected by electronic microscope/flow cytometry. At the same time G2/M retardation was founded. RT-PCR showed that PAB induced up regulation of Bax and down regulation of Bcl-2. Conclusion： In vitro PAB can inhibit the growth of SKOV3 cell line and induce apoptosis. The IC50 of 72h is 10μmol/L. Its mechanism may correlate with the up-regulation of bax and down-regulation of Bcl-2.

关 键 词：卵巢癌SKOV3细胞系 土荆皮酸 细胞凋亡 

分 类 号：R737.31[医药卫生—肿瘤] R284.1[医药卫生—临床医学]