对氨基苯磺酸生物降解动力学及降解机制研究  

Kinetics and Mechanism of 4-Aminobenzenesulphonate Biodegradation

作　　者：王艳青[1] 张劲松[1] 周集体[1] 侯玉琳[1] 周觅[1] 李俊芳[1] 

机构地区：[1]大连理工大学环境与生命学院,工业生态与环境工程教育部重点实验室,大连116023

出　　处：《环境科学》2009年第7期2136-2141,共6页Environmental Science

基　　金：辽宁省自然科学基金项目(20032122)

摘　　要：对菌株Pannonibactersp.W1在好氧条件下降解对氨基苯磺酸的动力学进行了研究,发现4-ABS初始浓度为中低浓度(50～1000mg/L)时,14h内几乎可以完全降解,符合一级降解动力学特征;初始浓度为高浓度(1200～2500mg/L)时,32h内4-ABS的降解率可达90%以上,且在降解初期符合零级降解动力学特征,而降解后期符合一级降解动力学特征.利用Haldane抑制模型能够很好地拟合不同4-ABS初始浓度下测得的比降解速率,得到模型参数分别为:最大比降解速率μmax=227.977mg/(g.h),饱和常数Ks=84.306mg/L,抑制常数Ki=1270.675mg/L.通过4-ABS降解过程中的紫外扫描和HPLC检测分析,以及菌株W1对不同苯系物的降解能力,说明4-ABS降解过程中几乎没有芳香类中间产物的积累,并初步推测了菌株W1降解4-ABS的代谢途径.Kinetics of 4-aminobenzenesulphonate （4-ABS） degradation by Pannonibacter sp. W1 under aerobic condition was studied. At medium and low initial concentration （50-1 000 mg/L）, the 4-ABS could be degraded almost completely in 14 h, and the degradation reaction conformed to one order kinetics. At high initial concentration （1 200-2 500 mg/L）, up to 90 % degradation rate could be achieved in 32 h, and it conformed to zero order kinetics in earlier period of degradation and one order kinetics in later period of degradation. Haldane inhibition model was used to fit the special degradation rate at different initial concentration,and the parameters μamx, Ks and Ki were determined to be 227.977 mg/（g·h）, 84.306 mg/L and 1270.675 mg/L, respectively. UV spectrum and HPLC detection indicated that no other aromatic intermediates were accumulated during the 4-ABS degradation. The degradation of other substituted benzenes by strain W1 were detected, and the metabolic pathway of 4-ABS degradation was proposed.

关 键 词：对氨基苯磺酸 生物降解 降解动力学 代谢途径 

分 类 号：X172[环境科学与工程—环境科学]