射干水煎液的致突变性检验及Ames测验的改进  

Mutagenicity of Belamcanda Chinesis Decoction and the Modification of Ames Test

作　　者：金建玲[1] 张辉[1] 刘波[1] 田晓[1] 高培基[1] 

机构地区：[1]山东大学生命科学学院微生物技术国家重点实验室,山东济南250100

出　　处：《中医药学报》2009年第3期12-16,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目资助(No.39430020)

摘　　要：目的:(1)检验射干水煎液的致突变性;(2)因经典的Ames测验体系在检验含组氨酸(蛋白质等)充分的样品时易出现假阳性结果,不适合中药体外致突变性检验,所以需要对Ames测验体系加以改进以适合中药等含组氨酸样品的体外致突变性检验。方法:通过经典的Ames测验检测射干的体外致突变性;通过哺乳动物骨髓细胞染色体畸变实验检测射干的体内染色体致畸作用;通过增设含补充组氨酸(含量对应于中药样品中组氨酸浓度)的阴性对照,排除样品中组氨酸成分对Ames测验结果的影响。结果:高浓度的射干水煎液在经典的Ames测验中为强阳性;在最高耐受浓度下哺乳动物骨髓细胞染色体致畸实验为阴性;用改进的Ames测验体系排除了药液中组氨酸成分的影响后,射干水煎液均为阴性。结论:经典的Ames测验不适合射干水煎液致突变性检测,改进后的Ames实验体系适合。射干水煎液在体外和体内均没有致突变性。Objective:To assay the mutagenicity and teratogenicity of Belamcanda chinesis(BC) decoction;modify the Ames test to make it suitable for evaluation the mutagenicity of herbal medicine samples.Methods:The mutagenicity of BC decoction was assayed by standard Ames test;the teratogenicity was assayed by mammalian bone marrow chromosomal aberration test.In the modified Ames test system,there included a group of additional negative control experiments-in each experiment,the test media was supplied with histidine(histidine amount equaled to the histidine in different concentration of EL decoction).Results:The mutagenicity of EL decoction was strong positive in the standard Ames test.The teratogenicity of EL decoction was negative result in mammalian bone marrow chromosomal aberration test.By the modified Ames tests-plate incorporation method,mutagenicity was negative.Conclusion:The standard Ames test was not suitable for evaluation the mutagenicity of EL decoction,but the modified Ames test was suitable.The mutagenicity and the teratogenicity of EL decoction were all negative.

关 键 词：射干 Ames测验 致突变性 染色体畸变 改进 

分 类 号：R285.5[医药卫生—中药学] R979.1[医药卫生—中医学]