实时荧光定量PCR技术在转基因检测中的应用  

Real-time quantitative PCR and the application in the detection of genetically modified plants

作　　者：敖金霞[1] 高学军[1] 仇有文[1] 郭士成[2] 

机构地区：[1]东北农业大学生命科学与生物技术研究中心,农业部转基因生物产品成分监督检测测试中心,哈尔滨150030 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2009年第6期141-144,共4页Journal of Northeast Agricultural University

基　　金：东北农业大学创新团队项目(CXT004-3-2)

摘　　要：实时荧光定量PCR技术是一种新型的核酸定性、定量检测、分析技术。随着转基因产品大规模商业化,转基因生物的环境安全和食用安全性问题引起了全球范围内广泛的争论和关注。实时监测、定量准确、灵敏度高、反应速度快、重复性好及PCR反应后不需电泳检测等优点,使RQ-PCR逐步成为转基因检测的重要工具。Real-time quantitative PCR is a newly developed technique for both nucleic acid analysis and quantitative measurement. As the development of the Genetically Modified ment and food result have been paied the whole world peoples＇ widely Organisms, the securities of the environattention. The merits of the real-time quantitative PCR, such as its good accur-acy, real-time, quickness, repeatability and there be no need to take the electrophoresis, enable it to be one of the most important tools in the check of the GMO.

关 键 词：实时荧光定量PCR TaqMan探针法 SYBR Green I染料法 转基因检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]