补肾化痰法对Alzheimer病模型大鼠行为学的影响及作用机制  

Effects and Function Mechanism of Replenishing Kidney-essence and Removing Phlegm Therapy on Behavior in the Rats of AD

作　　者：胡慧[1] 王平[1] 孔明望[1] 丁凤敏[1] 

机构地区：[1]湖北中医学院,武汉430061

出　　处：《成都医学院学报》2009年第2期79-82,85,共5页Journal of Chengdu Medical College

基　　金：国家中医药管理局基金项目(NO.04-05JP41);湖北省卫生厅科研基金项目(JX2B66)

摘　　要：目的观察补肾化痰法对AD模型大鼠行为学的影响并探讨其可能机制。方法应用脑立体定向技术给大鼠BNM注射凝聚态Aβ25-35+IBO构建AD模型。注射2周后,高、中、低量组分别给大鼠该药水煎液灌胃;西药组用哈伯因混悬液灌胃;正常组、空白组、模型组均给予等容积的生理盐水灌胃。28d后采用Morris水迷宫进行行为学评价。结果行为学结果显示高、中、低量组和西药组的平均潜伏期较模型组有明显缩短(P<0.01);高、中量组的象限百分比较模型组有明显提高(P<0.01),20%、40%区域较模型组有明显减少(P<0.01);模型组大鼠空间探索的象限百分比和跨过原平台位置次数比正常组和空白组明显降低(P<0.01)。西药组和高、中、低量组的象限百分比和跨过原平台位置次数较模型组有明显提高(P<0.01),并呈现一定量效关系(P<0.01)。结论补肾化痰法可显著改善AD模型大鼠的学习记忆障碍。Objective To observe effects and function mechanism of replenishing kidney-essence and removing phlegm therapy on behavior in the rat of AD. Methods Application of technology to stereotactie brain of rats injected BNM condensed Aβ25-35 ＋ IBO build AD model. 2 weeks after injection, high, medium and low volume of the rats were administered the drug decoction; western medicine group with Huperzine oral suspension; normal group, blank group, model group were given equal volume of normal saline ig. 28 days after the Morris water maze used for behavioral evaluation. Results Behavioral results showed high, medium and low volume group and western medicine group than the average incubation period of the model group was significantly shorter （P〈0.01）; high percentage of the volume group than the quadrant of the model group had markedly improved （P〈0. 01）, 20 %, 40% than the region has significantly reduced the model group （P〈0. 01）; model group the percentage of space exploration and the quadrant across the former platform position than the normal group and blank group were significantly lower （P 〈0. 01）. Western medicine group and the high, medium and low percentage and crossed the quadrant the original platform location of the volume group than in the model group had markedly improved （P〈0. 01）, and a certain dose-effect relationship （P〈0. 01 ）. Conclusion Bushen Huatan method（ replenishing kidney-essence and removing phlegm therapy） can significantly improve the AD model of learning and memory impairment in rats.

关 键 词：补肾化痰法 ALZHEIMER 行为学 

分 类 号：R592[医药卫生—老年医学]