家蚕27、28号染色体基因的生物信息学分析  

Bioinformatic Analysis of Genes on Chromosomes 27 and 28 in Bombyx mori

作　　者：段彪[1,2] 查幸福[1] 沈以红[1] 代方银[1] 夏庆友[1] 向仲怀[1] 

机构地区：[1]西南大学蚕学与系统生物学研究所,重庆400716 [2]西南大学荣昌校区,重庆402460

出　　处：《蚕业科学》2009年第2期241-247,共7页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(编号2005CB12-1000);国家自然科学基金项目(编号30800804)

摘　　要：利用家蚕基因组精细图分析第27、28号染色体上的基因结构及其编码蛋白的功能结构域,可为在家蚕资源库中鉴定这两条染色体上的突变基因以及发现新的形态标记提供重要线索。基因预测及结构分析显示:家蚕第27和28号染色体是基因数目最少的两条染色体,预测基因数目分别是241个和288个,平均每个基因分别具有7.7和5.3个外显子;蛋白质全长编码区序列(CDS)长度主要分布于400-800 bp之间;CDS的GC含量分别是49.72%和46.54%。蛋白质功能结构域分析发现,家蚕第27、28号染色体上基因所编码的蛋白分别具有76和92种结构域,其中最多的3种结构域分别为Sugar_tr、MFS_1、zf-C2H2和UDPGT、DUF、Serpin。将家蚕第27、28号染色体上的预测基因与果蝇突变基因进行同源检索的结果显示,家蚕第27、28号染色体上分别有16和15个基因具有明显的可能突变表型。With the completion of silkworm detailed genome sequences, we were able to analyze gene structure information and the coded proteins＇ functional domains on chromosomes 27 and 28. This kind of analysis can provide important clues for identifying mutant genes on the two chromosomes and acquiring new morphological markers. Analyses on gene structures showed that chromosomes 27 and 28 have 241 and 288 predictable genes respectively, the fewest among all chromosomes. And there are 7.7 and 5.3 exons per gene on average. Protein full-length coding sequence （CDS） is generally 400 -800 bp long. And the contents of GC are 49. 72% and 46.54% on chromosomes 27 and 28 respectively. Analyses on protein functional domains showed that the proteins coded by genes on chromosomes 27 and 28 possess 76 and 92 kinds of domains respectively. The top three domains on chromosome 27 are Sugar_tr, MFS_1 and zf-C2H2. Those on chromosome 28 are UDPGT, DUF and Serpin. Homology search was performed between the predicted genes of Bombyx mori and the mutant genes of Drosophila by BLASTX. The result showed that 16 and 15 genes on chromosomes 27 and 28 possess possible mutant phenetypes respectively.

关 键 词：家蚕 突变基因 27号连锁群 28号连锁群 生物信息学 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]