虾青素对ECV-304细胞的抗氧化作用及其作用机制  

Antioxidative action of astaxanthin in ECV-304 cells and its mechanism

作　　者：宋晓冬[1] 刘文波[1] 张丽霞[1] 杨成[1] 张瑾锦[1] 王秀文[1] 

机构地区：[1]滨州医学院实验中心

出　　处：《滨州医学院学报》2009年第3期161-163,166,共4页Journal of Binzhou Medical University

基　　金：山东省软科学研究计划项目(2008RKB185)

摘　　要：目的研究虾青素的抗氧化作用并探讨其作用机制。方法MTT、LDH释放实验检测了虾青素对ECV-304细胞活力的影响,超氧化物歧化酶(SOD)、二氨基二苯甲烷(MDA)、细胞内氧化应激活性氧(ROS)、一氧化氮(NO)的测量实验检测了虾青素对ECV-304细胞的抗氧化作用,ATP、细胞色素C氧化酶(COX)、线粒体跨膜电位的测量实验检测了虾青素对ECV-304细胞线粒体的保护作用。结果虾青素能保护细胞膜不受损伤,提高细胞活力,降低ROS、MDA的含量,升高SOD活性和NO含量。同时,虾青素还能显著升高ATP水平和COX活性,线粒体跨膜电位有所升高。结论虾青素的抗氧化作用可能是通过保护线粒体功能实现的。Objective To study astaxanthin antioxidative action in ECV-304 cells and its mechanism. Methods The effects of astaxanthin on cell viability were assayed by MTT and LDH release methods. The effects of astaxanthin on antioxidative action were examined by SOD activity and the contents of MDA, ROS and NO. The protective effects on mitochondria were checked by ATP level, COX activity and mitochondrial membrane potential. Results Astaxanthin could protect cell membrane, increase cell viability, SOD activity, NO content, ATP level, COX activity and mitochondrial membrane potential. Meanwhile Astaxanthin decreased the contents of ROS and MDA. Conclusion The effects of astaxanthin on antioxidative action might be completed through the pathway to protect the mitochondria.

关 键 词：虾青素 抗氧化作用 线粒体跨膜电位 ATP 

分 类 号：Q73[生物学—分子生物学]