产酯酶海洋微生物的筛选、鉴定及系统发育分析  

Screening,identification and phylogenetic analysis of marine microorganisms producing extracellular esterase

作　　者：郑鸿飞[1,2] 孙谧[1] 王跃军[1] 郝建华[1] 高强[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所海洋产物资源与酶工程实验室,青岛266071 [2]上海海洋大学食品学院,201306

出　　处：《渔业科学进展》2009年第3期68-73,共6页Progress in Fishery Sciences

基　　金：国家高技术研究发展(863)计划项目(2007AA091602);国际科技合作重点计划项目(2005DFA30830)共同资助

摘　　要：通过以三丁酸甘油酯和α-乙酸萘酯为底物,建立了一种快速、准确的酯酶产生菌的筛选方法,并从样品中分离到1株产酯酶活力和稳定性较高的菌株EB-1,对其进行了形态学、生理生化特征鉴定和16S rDNA序列分析。结果表明,该菌株与Bacillus subtilis subsp.subtilis模式菌株的同源性高达99.79%;又根据其产黑色素这一特性,最终将其确定为Bacillus subtilis var.niger,其16S rDNA序列在GenBank的注册号为EU016526。With α-naphthyl acetate and tributyrin as substrate,a precise and rapid screening method was developed. One stable strain-EB-1, which was isolated from related samples, showed the highest esterase-producing ability among the 281 strains. Through study on its morphological,physiological and biochemical characteristics as well as 16S rDNA sequence homolo- gy comparison,strain-EB-1 was identified as Bacillus subtilis vat. niger, which was almost the closest relative to the standard strain with 99.79 % similarity in sequence under the phylogenetic tree. The 16S rDNA sequence of strain-EB-1 has been deposited in GenBank,registration number was EU016526.

关 键 词：海洋微生物 酯酶 16S RDNA 系统发育学分析 

分 类 号：Q55[生物学—生物化学]