Survivin基因在急性胰腺炎中的表达及其意义  

Expression of survivin gene in a rat model of acute pancreatitis

作　　者：李震东[1] 马清涌[2] 罗羽宏[1] 

机构地区：[1]暨南大学附属第一医院肝胆外科,广东广州510630 [2]西安交通大学第一医院肝胆外科,陕西西安710061

出　　处：《南方医科大学学报》2009年第6期1141-1143,1146,共4页Journal of Southern Medical University

基　　金：广东省医学科研课题(A2008366)

摘　　要：目的探讨survivin基因在鼠急性胰腺炎中的表达及其意义。方法采用胰胆管逆行穿刺注射牛磺胆酸钠建立不同炎症程度的急性胰腺炎模型,应用免疫组化、Western blot及RT-PCR等方法检测survivin基因在胰腺组织中的表达,TUNEL法检测胰腺泡细胞凋亡的变化。结果正常胰腺组织无survivin的表达;建立急性胰腺炎模型后,随胰腺炎症程度的加重,Survivin蛋白和mRNA的表达水平逐渐升高,而腺泡细胞凋亡率则逐渐下降。结论Survivin蛋白不仅通过其抗凋亡作用参与了急性胰腺炎时胰腺腺泡细胞凋亡的调节,而且通过其抗凋亡作用使受炎症损伤的胰腺细胞由凋亡转为坏死,从而加重急性胰腺炎病情,可能是预测急性胰腺炎严重程度的一个重要指标。Objective To investigate the expression of survivin in acute pancreatitis in rats. Methods Acute pancreatitis was induced in rats by retrograde injection of sodium taurocholate into the pancreaticobiliary duct. The expressions of survivin in the pancreatic tissues was detected by immunohistochemisty, Western blotting and RT-PCR, and the apoptotic ratio of the acinar cells was determined by TUNEL assay. Results Survivin was not detected in the control group, and in rat models of acute pancreatitis, the expressions of survivin protein and mRNA increased but the apoptotic index of the acinar cells decreased gradually with the severity of inflammation, Conclusion Survivin is involved in the regulation of acinar cell apoptosis and also the necrosis of the apoptotic acinar cells through its anti-apoptosis activity to aggravate acute pancreatitis, suggesting its value as a promising marker in predicting theseverity of acute pancreatitis.

关 键 词：SURVIVIN 凋亡 急性胰腺炎 

分 类 号：R576[医药卫生—消化系统]