KDR-CDglyTK融合基因系统对结肠癌细胞增值的影响  

Effect of KDR recombinant adenovirus containing double suicide gene on the proliferation of human colon adneocarcinoma SW620 cells

作　　者：刘跃东[1,2] 汪森明[1] 黄宗海[1] 李强[1] 

机构地区：[1]南方医科大学珠江医院普外科,广东广州510282 [2]南方医科大学江都医院外科,广东广州510450

出　　处：《南方医科大学学报》2009年第5期887-889,893,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(013072)

摘　　要：目的探讨腺病毒介导KDR启动子驱动的融合基因体系对人结肠癌细胞SW620增值的影响。方法重组腺病毒AdEasy-KDR-CDglyTK体外感染表达KDR的SW620细胞株和对照组不表达KDR的LS174T细胞株,并给予不同浓度的前药GCV(ganciclovir)和/或5-FC(5-fluorocytosine),观察该体系对SW620细胞生长增值的影响。结果携带双自杀基因(CDglyTK)和报告基因(GFP)的重组腺病毒载体,感染复数为100时,95%以上的受感染SW620和LS174T细胞中有GFP表达。已转染腺病毒的SW620和LS174T细胞同未转染的各自细胞在细胞生长方面无显著性差异(P>0.05)。在前药应用下,已转染腺病毒的SW620和LS174T细胞表现出对前药不同的敏感性:表达KDR的SW620细胞对前药具有较高的敏感性,与前者相比,不表达KDR的LS174T细胞对前药不敏感(P<0.01)。融合基因的疗效优于单一自杀基因(P<0.01)。结论KDR基因启动子可以调控融合基因体系选择性地杀伤人结肠癌SW620细胞,抑制其增值。Objective To study the effect ofadenovirus （Ad）-mediated fusion gene system driven by the KDR promoter on the proliferation of human colon adneocarcinoma SW620 cells. Methods The KDR-expressing SW620 cells and LS174T cells not expressing KDR were both infected with AdEasy-KDR-CDglyTK followed by treatment with the prodrugs 5-FC and/or ganciclovir at different concentrations. The effect of the transfection on the cell proliferation was evaluated. Results The expression of green fluorescent protein （GFP） was observed in 95% of the infected SW620 and LS174T cells with a multiplicity of infection （MO1） of 100. Significant difference was not founded in the growth of SW620 and LS 174T cells with or without the transfection. The infected SW620 cells exhibit high sensitivity to the prodrugs, but the infected LS174T cells did not （P〈0.01）. The CDglyTK fusion gene produced much stronger killing effect of on the target cells than either of the single suicide genes （P〈0.01）. Conclusion CDglyTK fusion gene system driven by the KDR promoter selectively kills the KDR-CDglyTK SW620 cells and inhibits the cell proliferation.

关 键 词：人结肠癌细胞 KDR启动子 融合基因 自杀基因 腺病毒 更昔洛韦 5-氟胞嘧啶 

分 类 号：R735.35[医药卫生—肿瘤]