黄芪多糖诱导K562细胞γ-珠蛋白基因表达  

Astragalus polysaccharides-induced γ-globin mRNA expression in K562 cells

作　　者：黄为民[1] 钱新华[1,2] 赵丹华[1] 

机构地区：[1]南方医科大学南方医院新生儿科,广东广州510515 [2]南方医科大学江都医院儿科,广州510450

出　　处：《南方医科大学学报》2009年第5期939-942,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(7005146)

摘　　要：目的探讨黄芪多糖(APS)对K562细胞γ-珠蛋白基因表达的诱导作用。方法以K562细胞为模型,以APS诱导的细胞为实验组,未加药细胞为空白对照,丁酸钠(NaB)处理的细胞为阳性对照,分别用联苯胺染色和RT-PCR分析联苯胺染色阳性率、Aγ-和Gγ-珠蛋白基因mRNA表达水平。结果(1)与空白对照组相比,300mg/LAPS诱导48h后联苯胺染色阳性率由(4.37±0.58)%升高至(15.67±1.80)%(P<0.05)。300mg/LAPS诱导K562细胞48h的联苯胺染色阳性细胞总数为(60.40±6.33)×102,与NaB组(42.02±16.42)×102相比差异有显著性意义(P<0.05)。(2)与空白对照组相比,300mg/LAPS诱导48hAγ和Gγ珠蛋白基因mRNA表达分别增加3.59±0.16倍和5.02±0.81倍(P=0.000)。结论APS可诱导K562细胞Aγ和Gγ珠蛋白基因mRNA表达增强,具有治疗β-珠蛋白生成障碍性贫血的潜能。Objective To investigate the effects ofAstragalus polysaccharides （APS） in inducing the mRNA expression of ^Aγ- and ^Gγ-globin in K562 cells. Methods K562 cells were treated with APS at the concentration of 150, 300, and 450 mg/L, with Na-buytrate （NaB）-treated cells serving as the positive control and untreated cells as the blank control. Benzidine staining was used to examine the changes in hemoglobin synthesis in K562 cells after the treatments, and RT-PCR was employed to investigate the mRAN expression of ^Aγ- and ^Gγ-globin. Results Compared with the untreated cells, APS treatment （300 mg/L） for 48 h resulted in a significant increase of the percentages of benzidine-positive cells from （4.37±0.58）% to （15.67±1.80）%, and also in significantly increased expression of ^Aγ-globin and ^Gγ-globin mRNAs by 3.59 ±0.16 and 5.02 ±0.81 folds, respectively （P=0.000）. Conclusion APS potently enhances the mRNA expression of ^Aγ- and ^Gγ-globin in K562 cells and warrants further evaluation as a potential therapeutic agent for β-thalassemia.

关 键 词：黄芪多糖 Β-珠蛋白生成障碍性贫血 K562细胞 γ珠蛋白基因 丁酸钠 

分 类 号：R285.5[医药卫生—中药学]