急性肠系膜上动脉栓塞动物模型的建立  

The establishment of the animal model of acute superior mesenteric artery embolization

作　　者：马永强[1] 杨宁[2] 张晓峰[3] 路军良[4] 

机构地区：[1]北京大学第三医院放射科,100083 [2]北京协和医院放射科 [3]北京妇产医院放射科 [4]北京军区总医院放射科

出　　处：《介入放射学杂志》2009年第6期466-469,共4页Journal of Interventional Radiology

摘　　要：目的制作一种适合后续去栓治疗的急性肠系膜上动脉栓塞动物模型。方法8只杂种犬,体外制作动物自体血栓,采用经皮股动脉穿刺入路,选择性插入8F动脉长鞘至犬肠系膜上动脉主干后注入自体血凝块,造影复查直至肠系膜上动脉主干完全闭塞。分别在栓塞后2、4、6和8h于肠系膜上动脉开口部造影复查,观察自体血栓是否稳定,肠系膜上动脉有无自行再通,并经腹部切口探察,取病理活检。结果8只实验犬均成功建立急性肠系膜上动脉栓塞致肠缺血模型。大体观察及镜下可见随栓塞时间的延长,肠管损伤逐渐加重,组内一致性良好。结论采用本实验方法可成功建立适合后续去栓治疗研究的急性肠系膜上动脉栓塞动物模型。Objective To establish an animal model of acute superior mesenteric artery embolization suitable for follow-up embolectomy study. Methods Via the femoral artery approach selective catheterization of the main branch of superior mesenteric artery with a 8 F-guiding catheter was performed in 8 hybrid dogs, which was followed by the injection of auto-clots prepared in vitro until the main branch of superior mesenteric artery was completely occluded, which was confirmed with angiography. Follow-up angiography of superior mesenteric artery was carried out 2, 4, 6 and 8 hours after the procedure to check the stability of the auto-clots and the embolization, Intraperitoneal explore through abdominal incision was performed and abnormal bowel was taken out for pathologic examination. Results The acute superior mesenteric artery embolization model was successfully established in all 8 dogs. Intestinal ischemia was found in all of the 8 dogs. Macropathologically and microscopically, the intestinal isehemic damage deteriorated gradually with time. Conclusion lnterventional technique can successfully establish appropriate animal model of acute superior mesenteric artery embolization, which is suitable for the follow-up embolectomy study.

关 键 词：肠系膜上动脉 栓塞 动物模型 

分 类 号：R-332[医药卫生]