低氧预适应对自体原位肝移植大鼠肠黏膜损伤的影响  

Hypoxia precondition effects on injury of intestinal mucous membrane in autologous orthotopic liver transplantation rat

作　　者：刘新颜[1] 张培建[1] 冯敏[1] 陶立德[1] 李勇[1] 周斌[1] 金成[1] 

机构地区：[1]扬州大学第二临床医学院普通外科研究室,江苏扬州225001

出　　处：《中国现代医学杂志》2009年第11期1625-1628,共4页China Journal of Modern Medicine

基　　金：江苏省卫生厅医学科研项目(No:H200770)

摘　　要：目的研究低氧预适应对肝移植大鼠肠黏膜损伤的影响。方法建立SD大鼠自体原位肝移植模型,随机分为3组,A组:低氧预适应自体原位肝移植组(n=40),B组:自体原位肝移植组(n=40);C组:假手术组(n=8)。A、B组均在术后1、6、12、24和48h各取8只处死,取小肠组织做MDA检测,HE染色切片观察显微结构的变化,电镜观察超微结构的变化,免疫组织化学染色切片应用MiVnt显微图象分析系统观察肠组织IgA浆细胞数量的变化。结果A组术后各时段肠黏膜损伤较B组术后相应时段轻,C组变化不明显。结论低氧预适应对大鼠无肝期肠道淤血性缺氧诱导的肠道黏膜损伤有保护作用。[Objective] To study the effects of hypoxic preconditioning on intestinal mucosal barrier in the course of the liver transplantation. [Method] SD rats were randomly assigned into three groups, Group A： hypoxic preconditioning transplantation group （n =40）; Group B： autologous orthotopic liver transplantation group （n =40）; Group C： sham-operated group （n =8）. Autologous orthotopic liver transplantation model has been established. When the operation were successfully and reperfused for different times, liver samples and intestina parva were obtained from different experiment groups. The MDA in jejunum of rats were analyzed after liver transplantation in rats. The hepatic function was demonstrated by the level of serum ALB, TBIL, ALT, AST, and ALP. The MDA were determined in serum of recipients after liver transplantation as well. The morphological changes of caudate lobe of liver and intesti- nal epithelial basement membrane were observed by optics microscopy and transmission electron microscopy, analyzed by Mi Vnt image analysis system. [Results] The intestinal mucosa injury of all time corresponding to liver transplantation is more light in group A than in group B. [Conclusions] Hypoxic preconditioning had protective effects on injury of intestinal mucosa caused by congestion hypoxia during liver transplantation course.

关 键 词：低氧预适应 肝移植 缺血再灌注损伤 肠黏膜屏障 

分 类 号：R365[医药卫生—病理学]