自噬在ALA-PDT诱导C6胶质瘤细胞死亡中的作用机制  

Effects of autophagy on ALA-PDT induced C6 glioma cells death

作　　者：肖虹[1] 冯华[1] 程茗[2] 李梅[1] 杜鹏[1] 程迎新[1] 

机构地区：[1]第三军医大学西南医院神经外科,重庆400038 [2]第三军医大学大坪医院眼科

出　　处：《立体定向和功能性神经外科杂志》2009年第3期148-151,共4页Chinese Journal of Stereotactic and Functional Neurosurgery

基　　金：国家自然科学基金项目(编号:30670506);重庆市自然科学基金重点项目(编号:2008BA5002)

摘　　要：目的探讨自噬在光敏剂5-氨基乙酰丙酸(aminolaevulinic acid,ALA)介导的光动力治疗(photo-dynamic therapy,PDT)C6胶质瘤细胞死亡中作用机制。方法ALA-PDT处理后不同时间,用自噬特异染料MDC和免疫荧光法观察自噬水平变化,Western blotting检测自噬相关蛋白cathepsin B的表达水平。结果MDC染色和免疫荧光观察可见ALA-PDT后C6胶质瘤细胞内有大量含点状荧光颗粒的自噬泡和自噬体标志物LC3绿色荧光蛋白,Western-blot示自噬相关蛋白cathepsin B表达水平明显升高,其自噬水平随ALA-PDT后时间而逐渐增强。结论ALA-PDT可诱导C6胶质瘤细胞发生自噬,其诱导胶质瘤细胞发生自噬的机制可能与溶酶体酶cathepsin B大量释放有关。Objective To study the effects and the mechanisms of autophagy on photosensitizer Aminolaevulinic acid（ALA）--mediated--photodynamic--therapy induced death of C6 glioma cells. Methods After treatment with ALA--PDT, MI）C staining and immunofluorescenee were used to examine autophagy induced by AI.A--PDT in C6 glioma cells. The expression levels of autophagy--associated--protein cathepsin B were measured by Western blot. Results ALA-- PDT stimulated the autophagic vacuole and LC3 activity as evidenced by the appearance of punctuate monodansylcadaverine （MDC） and immunofluorescence staining. The expression levels of cathepsin B were elevated. The intensity of autophagy was correlated with treatment time of ALA--PDT in C6 glioma cells. Conclusion ALA--PDT may induce autophagy in C6 glioma cells. The autophagy mechanism of ALA--PDT induced C6 glioma cells may be related to the release of lysomal enzyme cathepsin B.

关 键 词：5-氨基乙酰丙酸 光动力治疗 C6胶质瘤细胞 自噬 

分 类 号：R739.41[医药卫生—肿瘤]