重组人碱性成纤维细胞生长因子-自固化磷酸钙与医用胶原膜联合治疗牙周骨缺损的实验研究  

Treatment of periodontal defect with combination of GTR,CPC and bFGF:An experimental study in rabbits

作　　者：朱建华[1] 王颖[1] 曲晓娟[2] 

机构地区：[1]佳木斯大学口腔医学院牙周黏膜病科,黑龙江佳木斯154002 [2]大连大学医学院口腔系,辽宁大连116622

出　　处：《牙体牙髓牙周病学杂志》2009年第6期323-326,362,共5页Chinese Journal of Conservative Dentistry

基　　金：大连市科技计划项目(2004B3SF152)

摘　　要：目的:评价重组人碱性成纤维细胞生长因子(rh-bFGF)与自固化磷酸钙(CPC)复合物和医用胶原膜联合应用对家兔牙周组织再生的作用。方法:选用16只家兔,在双侧下颌中切牙远中手术制备3.5 mm×2.5 mm×2 mm的"U"型骨缺损,采用3种不同方法进行处理,实验组1:rh-bFGF和CPC复合物植入,胶原膜覆盖;实验组2:CPC植入,胶原膜覆盖;对照组:单纯胶原膜覆盖。根据影像学和组织学测量指标对治疗结果进行评价。采用SPSS 13.0统计软件进行方差分析处理。结果:术后8周,所有"U"型骨缺损均有数量不等的新生牙槽骨、牙骨质和结缔组织。其中2个实验组的新生牙槽骨与对照组相比有显著差异(P<0.01),实验组1与实验组2相比也有显著性差异(P<0.05)。未出现根吸收和根固连等牙周再生治疗常见的不良结局。结论:应用rh-bFGF/CPC复合物更有利于牙槽骨再生。AIM： To evaluate the effectiveness of combined use of rh - bFGF, CPC and medical collagen membrane compounds on regeneration of the rabbit periodontal tissue. METHODS ： Sixteen rabbits were selected and bone defect of 3.5mm × 2.5mm × 2mm in “U” size was created in the distal area of the bilateral middle incisors of the mandible. As to the restoration methods, rh - bFGF and CPC compounds were implanted in the bone defect area in the group 1 and only the CPC was implanted in the group 2, which were both overlaid with the collagen membrane. There was only collagen membrane overlaying in the bone defect area in the control group. The therapeutic results were evaluated by clinical and imaging examination as well as histological index. SPSS13.0 software package was used for ANOVA. RESULTS ： 8 weeks after operation, there were various amounts of newly formed alveolar bone, cement and connective tissues in the dental defect of “U” size in all three groups. There was significant difference in alveolar osteogenesis between the control group and the 2 experimental groups. The difference was also significant between the two ex- perimental groups （P 〈 0.01 ）. CONCLUSION： Application of rh- bFGF/CPC compounds is beneficial to the regeneration of the alveolar bone and avoid the side effects such as root resorption and bone malunion, which often occur after the conventional periodontal regenerative therapy.

关 键 词：碱性成纤维细胞生长因子 人工合成骨材料 引导组织再生 牙周再生 

分 类 号：R780.2[医药卫生—口腔医学]