相溶解度法测定β-环糊精与4种黄酮类苷元的超分子包合常数  

Delermination of Supermolecular Inclusion Constants of β-cylcodextrin and 4 Flavonoid Aglycones by Phase Solubility Method

作　　者：徐涛[1] 潘见[2] 

机构地区：[1]合肥学院生物与环境工程系,安徽合肥230022 [2]合肥工业大学农产品生物化工教育部工程技术中心,安徽合肥230009

出　　处：《安徽农业科学》2009年第18期8637-8638,8695,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金重点项目(20136020);安徽省教育厅自然科学研究项目(KJ2008B229);合肥学院人才引进项目(RC058)

摘　　要：[目的]探讨β-环糊精促进黄酮类化合物色谱分离的可能性。[方法]以银杏黄酮中的4种苷元作为研究对象,研究β-环糊精对结构类似物在溶液条件下的包合作用,通过相溶解度法测定β-环糊精与不同黄酮苷元之间的相互作用。[结果]β-环糊精与槲皮素、木犀草素、山柰酚和异鼠李素的超分子包合常数分别为442.42、1 088.17、187.47、278.24 L/mol,说明β-环糊精可以高效选择性地结合一些黄酮,从而提高色谱分离度。[结论]β-环糊精可以尝试作为液相色谱流动相添加剂使用,提高不同黄酮苷元之间的分离度。[ Objective ] Aimed to investigate the possible contribution of β-eyelodextrin to chromatographic separation of flaovonoid. [Method] By choosing 4 flavonoid aglycones from Ginkgo biloba extract as model compounds, the inclusion phenomena of β-cylcodextrin on structural analogs under the solution condition was studied. The inclusion interaction of β-cylcodextrin with different flavonoid aglycones was determined by phase solubility analysis. [Result] Supermolecular inclusion constants of β-cylcodextrin with quercetin, luteolin, kaempferol and isorhamnetin were calculated as 442.42, 1088.17, 187.47,278.24 L/mol, indicated that through high selectivity combine some flavonoid , β-cylcodextrin can improve chromatographic separation selectivity. [Conclusion]β-cylcodextrin was suggested to add to mobile phase of HPLC, which could enhance the resolution of different flavonoid aglycones.

关 键 词：相溶解度法 Β-环糊精 黄酮苷元 超分子包合常数 

分 类 号：O656[理学—分析化学]