中药五倍子化学成分对早期龋再矿化作用的扫描电镜观察  

Effect of Chemical Compounds of Galla chinensis on Remineralisation of Initial Enamel Carious Lesions by SEM in vitro

作　　者：楚金普[1] 郝玉庆[2] 李继遥[2] 周学东[2] 

机构地区：[1]郑州大学口腔医学院,河南郑州450052 [2]口腔生物医学工程教育部重点实验室,四川大学,四川成都610041

出　　处：《时珍国医国药》2009年第6期1306-1307,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金重点资助项目(No.30430800);河南省教育厅自然科学研究计划项目(No.2008B320016)

摘　　要：目的观察pH循环后中药五倍子化学成分对早期龋表面形态变化的影响,了解中药五倍子化学成分对人工早期釉质龋的再矿化作用。方法应用离体牛切牙制备人工釉质龋标本60例,随机分为6组:NaF组(阳性对照)、GCE组、GCE-B组、GCE-B1组、GCE-B2组和去离子水组(阴性对照),进行体外pH循环实验后,各组样本分别放入2.5%戊二醛液内固定24 h,4℃,临界点干燥,镀金,扫描电镜下观察。结果再矿化后,NaF组的釉质表面变的比矿化前更加规则、均匀的外观,有许多圆形或椭圆形的晶体颗粒沉积,大颗粒常由数个小颗粒组成,密集、紧奏,略显隆起。GCE组、GCE-B组、GCE-B1组的釉质表面有一层似膜状的结构形成,有一定厚度,进一步观察这层膜结构是由一些呈小球状或不规则的颗粒组成,但外观显得相对不如氟化物组那样规则、均匀。而GCE-B2组釉质表面没有明显的沉积物形成,其外观与去离子水组相似,可见釉柱中心发生脱矿所形成的凹坑。结论五倍子化学成分GCE、GCE-B和GCE-B1具有潜在的促进早期龋再矿化的功效。Objective To evaluate the effect of chemical compounds of Galla Chinensis on the remineralisation of initial enamel carious lesions by scanning electron microscope in vitro. Methods Sixty bovine enamel blocks with early lesions were prepared and randomly divided into six treatment groups. The lesions were subjected to the pH - cycling regime for 12 days. Each daily cycle included 4 × 1 min applications with one of six treatments ; 1,000ppm F aq. （ as NaF, positive control） ; deionized water （ negative control） ; or 4,000 ppm aqueous solutions of four Galla Chinensis extracts （GCEs） ; GCE, GCE - B, GCE - B1, or GCE - B2. After pH - cycling, the enamel specimens were observed by scanning electron microscope. Results The results showed that there were sediments formed on the surface of carious lesion for the positive control, GCE, GCE - B and GCE - B1 groups except the GCE - B2 group and DDW group. Conclusion Galla Chinensis might be a promising source of non - fluoride anti - caries agents.

关 键 词：五倍子 有效成分 早期龋 再矿化 扫描电镜 

分 类 号：R285.5[医药卫生—中药学]