肺岩宁冲剂对肺癌细胞移植瘤组织基因差异表达的影响  

Effect of Genes Expression of Tumor Tissues in Node Mice Treated with Feiyanning Granulae by Microarray

作　　者：王菊勇 金叶[2] 徐振晔 秦胜营[3] 贺林[3] 

机构地区：[1]上海中医药研究院中医肿瘤研究所,200032 [2]上海大学生命科学学院 [3]上海交通大学Bio-X中心

出　　处：《肿瘤防治研究》2009年第6期470-474,共5页Cancer Research on Prevention and Treatment

基　　金：上海市浦江人才计划基金资助项目(07pj14079);上海市重点优势学科建设基金资助项目(Y0302)

摘　　要：目的应用基因芯片技术研究肺岩宁冲剂对裸鼠人肺癌细胞移植瘤组织的基因表达谱的影响,初步探讨肺岩宁冲剂抗肺癌的分子机制。方法将人肺癌细胞H460皮下移植瘤裸鼠24只随机分为三组:空白对照组:0.9%氯化钠溶液0.4ml灌胃×14天;化疗组:第1、3、5天DDP溶液1ml(含DDP0.1mg)腹腔注射,0.9%氯化钠溶液0.4ml灌胃×14天;肺岩宁冲剂组:肺岩宁冲剂药液0.4ml(含生药0.6g)灌胃×14天。治疗后第15天提取各组移植瘤组织的总RNA并与人全基因组芯片杂交,经激光共聚焦扫描仪扫描及LuxScan3.0软件分析后比较各组中的差异表达基因。结果与对照组比较,化疗组共筛查出差异表达基因2187条,其中上调基因997条,下调基因1190条,功能未知基因326条。肺岩宁冲剂组共筛查出差异表达基因2093条,其中上调基因968条,下调基因1025条以及未知功能基因145条。按照基因功能可分为细胞周期相关基因、代谢相关基因、细胞信号转导分子、转录调控因子等。结论为明确肺岩宁冲剂抗肺癌的分子机制提供重要参考。Objective To investigate the molecular mechanism on genomic level by using Fei-Yan-Ning Granulae in treating transplanted human lung carcinoma in nude mice. Methods Nude mice with transplanted human lung carcinoma H460 tumor were randomly divided into three groups： control group, chemotherapy group and Feiyanning Granulae treated group（FYN group）. The total RNA were isolated from carcinoma tissue of groups, and the changes of genes expression profile of tumor tissues were evaluated by cDNA microarray technology. Results Compared with the control group,the chemotherapy group showed 2187 genes changes. The 997 genes were ulyregulated and 1190 genes were down-regulated. The FYN group showed 2093 genes changes in their gene expression profiles. The 968 were up-regulated and 1025 gene were down-regulated, involving multiple scopes as cell cycle, signal tranduction, energy metabolism,protein synthesis,cell receptors as well. Conclusion The antitumor effect of Feiyanning granulae is multi-targeted, which elucidates part of the pharmacologic mechanism of Feiyanning granulae in molecular level.

关 键 词：基因芯片 肺癌 肺岩宁 中剂 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]