3种玻璃化冷冻液对水牛MII期卵母细胞冷冻-解冻后体外发育的影响  

Effects of three Types of Vitrified Solutions on in Vitro Development of Vitrified-Thawed MII Oocytes in Buffalo

作　　者：杨素芳[1] 陈玉[1] 冯贵雪[2] 王晓丽[1] 吴正三[1] 石德顺[1] 

机构地区：[1]广西大学动物繁殖研究所,南宁530005 [2]广西壮族自治区妇幼保健院生殖中心,南宁530003

出　　处：《基因组学与应用生物学》2009年第3期493-497,共5页Genomics and Applied Biology

基　　金：国家863计划资助项目(2005AA206130);广西青年基金项目(桂科青0542022)共同资助

摘　　要：本试验主要探索了3种玻璃化冷冻液对水牛MII期卵母细胞冷冻-解冻后体外发育的影响。水牛MII期卵母细胞经3组玻璃化冷冻液(Ⅰ:20%乙二醇(EG)+20%二甲基亚砜(DMSO);Ⅱ:20%EG+20%丙二醇(PROH);Ⅲ:20%EG+20%PROH+10%DMSO)毒性试验后,Ⅰ、Ⅱ和Ⅲ组体外受精的分裂率、8-细胞率和囊胚率之间差异不显著(P>0.05),分裂率均显著低于对照组(P<0.05),但Ⅱ组8-细胞以后的发育潜力跟对照组无显著差异(P>0.05)。进一步比较了3组玻璃化冷冻液对卵母细胞的玻璃化冷冻效果。卵子解冻后进行体外受精后,Ⅰ、Ⅱ和Ⅲ组的发育潜力均显著低于对照组(P<0.05),各组的8-细胞率和囊胚率之间无显著差异(P>0.05),但Ⅱ组卵裂率明显高于Ⅰ组(24.8±4.6%vs12.7±1.5%,P<0.05)。结果表明,3种冷冻玻璃化保护液均可用于冷冻水牛MII期卵母细胞,其中Ⅱ组处理的卵母细胞体外受精效果相对较好。The study was to investigate the effects of three types of vitrified solutions on in vitro development of vitrified-thawed metaphase Ⅱ （MⅡ） oocytes in buffalo. The toxicity of three types of vitrified solutions （Ⅰ： 20% ethylene glycol （EG）＋20% dimethyl sulfoxide （DMSO）; Ⅱ： 20% EG＋20% 1,2-propanediol （PROH）; Ⅲ： 20% EG＋20% PROH＋10% DMSO） was tested. There was no significant difference among three groups in cleavage rate, 8-cell rate and blastocyst rate （P〉0.05）, and the cleavage rate all were lower than that in the control （P〈0.05）. However, the developmental potential after 8-cell stage in group Ⅱ resembled with control （P〉0.05）. Moreover, the effect of different vitrified solutions on oocyte vitrification was investigated. The developmental potential of IVF was also lowered than that in the control （P〈0.05）, there was no siginficant difference among three groups in 8-cell rate and blastocyst rate （P〉0.05）, whereas the cleavage rate in group Ⅱ was significantly higher than that in group Ⅰ （24.8_＋4.6% vs 12.7±1.5%, P〈0.05）. In conclusion, the three vitrified solutions all could be used to vitrified buffalo MⅡ oocytes, but a better in vitro fertilization effect could be received when the buffalo MⅡ oocytes were vitrified by 20% EG＋20% PROH.

关 键 词：水牛 卵母细胞 玻璃化冷冻液 体外受精 

分 类 号：S823[农业科学—畜牧学]