利迪链霉菌A02抗真菌活性产物的分离和结构鉴定  

Extraction and structural identification of the antifungal metabolite of Streptomyces lydicus A02

作　　者：隋勤[1,2] 刘伟成[1] 卢彩鸽[1] 刘霆[1] 裘季燕[1] 刘学敏[2] 

机构地区：[1]北京市农林科学院植物保护环境保护研究所,北京100097 [2]东北农业大学植保系,哈尔滨150030

出　　处：《生物工程学报》2009年第6期840-846,共7页Chinese Journal of Biotechnology

基　　金：国家自然科学基金项目(No.30671346);北京市自然科学基金项目(No.6072010);北京市科技计划项目(No.D0706005040231)资助~~

摘　　要：利迪链霉菌A02是从京郊森林土壤中分离筛选出的植物病原真菌高效拮抗菌株。为了明确其抑菌活性的物质基础,利用大孔树脂和硅胶吸附柱层析、HPLC循环制备分离等方法,从菌株A02发酵液中分离获得了纯度达99.845%以上的单一组分活性化合物。经紫外光谱、高分辨质谱、红外光谱和核磁共振谱的测定和解析,确定了该活性化合物的分子量为665,分子式为C33H47NO13,化学结构与四烯大环内酯类抗生素纳他霉素相同。这一结果揭示了利迪链霉菌产生抗真菌天然产物的新功能,并为纳他霉素在植物病害生物防治中的应用开拓了新的途径。We isolated a high efficient antifungal strain A02 from forest soil in a suburb of Beijing. The result of polyphasic taxonomy confirmed that strain A02 belongs to Streptomyces lydicus. The fermented broth of the strain presented a stable and strong inhibiting activity against many plant pathogenic fungi. The purpose of this study was to ascertain the substance base of the antifungal activity of strain A02. We extracted the antifungal metabolite of A02 by using column chromatography with X-5 macroporous resin and 100-200 mesh silica gel respectively, and then purified it by LC-9101 recycling preparative HPLC with a SP-120-15 column （JAIGEL-ODS-AP）. An active compound with purity over 99.845% was finally obtained. The chemieal structure of the active compound was determined with spectroscopy methods, including ultraviolet spectrometry, infrared spectrometry, high resolution mass spectrometry and nuclear magnetic resonance. According to the analysis results, we identified the active compound as a tetraene macrolide antibiotic with the molecular weight of 665, the molecular formula C33H47NO13 and the same chemical structure as natamycin. Our research revealed a new biosynthetic function for S. lydicus to produce natamycin, and an expanding application field for natamycin to be used for the control of fungal plant diseases.

关 键 词：利迪链霉菌 活性产物 纳他霉素 病原真菌 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q93[农业科学—植物保护]