5-ASA对TNBS结肠炎大鼠血中性粒细胞凋亡及血清IL-8水平的影响  

Effect of 5-ASA on serum level of IL-8 and PMN apoptosis in TNBS-induced rat colitis

作　　者：贺文成[1] 李瑾[1] 周瑞[2] 唐平飞[1] 

机构地区：[1]武汉大学中南医院消化内科湖北省肠病医学研究中心,湖北省武汉市430071 [2]泰兴市人民医院消化内科,江苏省泰兴市225400

出　　处：《世界华人消化杂志》2009年第13期1297-1302,共6页World Chinese Journal of Digestology

摘　　要：目的:观察5-ASA对三硝基苯磺酸(TNBS)灌肠诱发的结肠炎大鼠血中性粒细胞凋亡及血清IL-8水平的影响,并探讨5-ASA治疗炎症性肠病(IBD)的作用机制.方法:成年SD大鼠30只,♀,随机分成A、B、C组,A组为正常对照组,B、C组采用TNBS/乙醇灌肠制作大鼠结肠炎模型.B组每天给予生理盐水1mL灌肠,C组每天给予5-ASA溶液(200mg/kg)灌肠,连续7d.经过处理后评定各组大鼠一般状况、结肠大体损伤及结肠组织学损伤;在全麻下心脏采血,分别通过流式细胞术检测血中性粒细胞凋亡率及通过酶联免疫吸附法检测血清中IL-8浓度.结果:与A组比较,B组的DAI评分、大体损伤评分和组织学损伤评分及血清IL-8水平均显著升高(2.74±0.437分vs0.27±0.346分,5.10±1.101分vs0.50±0.527分,4.70±0.949分vs0.44±0.458分,720.97±71.718ng/Lvs129.88±18.399ng/L,均P<0.01),而C组(1.34±0.385分,1.70±0.483分,1.50±0.850分,392.84±43.628ng/L)明显低于B组(均P<0.01);与A组比较,B组中性粒细胞凋亡率明显降低(30.54%±4.036%vs56.13%±5.188%,P<0.01),而C组(48.89%±4.522%)明显高于B组(P<0.01).结论:5-ASA灌肠对结肠炎大鼠具有显著的治疗作用,其机制可能与其降低血清IL-8水平及诱导血中性粒细胞凋亡有关.AIM： To investigate the therapeutic mechanism of 5-ASA in inflammatory bowel disease （IBD） through observation of its effects on serum level of IL-8 and PMN apoptosis in trinitrobenzene sulphonic acid （TNBS）- induced rat colitis. METHODS： Thirty female Sprague-Dawley （SD） rats were randomly divided into three groups, namely, normal control group （group A） and colitis groups （group B and C）. Then group B was given 1 mL normal saline while group C was given 5-ASA （200 mg/kg） for 7 d. All of the animals in three groups were anesthetized, and the macroscopic and histological changes of the colon were evaluated and scored. Expres-sion of serum IL-8 was detected using ELISA, and the apoptosis of PMN was examined by flow cytometry. RESULTS： Compared with group A, macroscopic and histological scores and the serum level of IL-8 were significantly higher in group B （2.74 ± 0.437 vs 0.27 ± 0.346, 5.10 ± 1.101 vs 0.50 ± 0.527, 4.70 ± 0.949 vs 0.44 ± 0.458, 720.97 ± 71.718 ng/L vs 129.88 ± 18.399 ng/L, all P 〈 0.01）, but they were markedly decreased in group C than in group B （1.34 ± 0.385 vs 2.74 ± 0.437,1.70 ± 0.483 vs 5.10 ± 1.101, 1.50 ± 0.850 vs 4.70 ± 0.949, 392.84 ± 43.628 ng/L vs 720.97 ± 71.718 ng/L, all P 〈 0.01）. Compared with group A, PMN apoptosis rate was notably lower in group B （30.54% ± 4.036% vs 56.13% ± 5.188%, P 〈 0.01）, but it was markedly increased in group C （48.89% ± 4.522%） than in group B （P 〈 0.01）. CONCLUSION： There is notably therapeutic effect in topical enema with 5-ASA for IBD, which may be related to reduced serum level of IL-8 and induced PMN apoptosis.

关 键 词：炎症性肠病 三硝基苯磺酸 5-氨基水杨酸 白介素-8 中性粒细胞 凋亡 

分 类 号：R574.62[医药卫生—消化系统]