MTT比色法抗肿瘤药物筛选实验条件和数据优化探索  

Optimization of Experimental Conditions and Data Processing of in vitro MTT Colorimetric Assay for Anti-tumor Drug Screening

作　　者：黄银久[1,2] 宋宝安[2] 金林红[2] 胡德禹[2] 陈卓[2] 杨松[2] 李宁[2] 吴守伟[1] 

机构地区：[1]蚌埠医学院生物科学系,安徽蚌埠233030 [2]贵州大学精细化工研究开发中心 “绿色农药与生物工程”教育部重点实验室,贵州贵阳550025

出　　处：《激光生物学报》2009年第3期401-408,共8页Acta Laser Biology Sinica

基　　金：国家“973”计划项目(2003CB114404);科技部重点计划项目(2005DF100660);安徽省高校省级自然科学研究项目(KJ2008B97ZC)

摘　　要：MTT比色法是一种重要的体外抗肿瘤药物筛选方法。以PC-3细胞系为研究对象，对影响MTT比色法抗肿瘤药物筛选实验的主要因素-细胞密度、实验操作环节、OD值选取、以及数据的优化处理进行实验探讨。结果表明，当检测化合物6h．72h的抑制活性时，种细胞密度2000个／孔为宜。同时，采用本文所提的实验条件和数据处理办法，可以实现实验结果的准确可靠，3次测试偏差不超过10％。MTT colorimetric assay is an important method for anti-tumor drug screening. The effects of cell density, experimental operation, OD value and data processing on MTT colorimetric assay were investigated on PC-3 cell line. It was found that 2 000 cells/well of plating cell density is appropriate for testing compound inhibition activity within 6 h 72 h; furthermore, optimization of experimental result can be achieved through the methods of experimental condition and data processing mentioned in this paper. For instance, the deviation of three independent experiments is not more than 10 %.

关 键 词：MTT 比色法 实验条件 数据 优化 

分 类 号：R733.7[医药卫生—肿瘤]