Comparison and Evaluation of Several Dermatophagoides Pteronyssinus Allergen Extracts for Skin Prick Test  

作　　者：Jin-lu Sun Rui-qi Wang Jia Yin Liang-lu wang Hong-yu Zhang Hong Li Yu-xiang Zhi Li-ping Wen Kai Guan Jian-qing Gu Hai-juan He Ying Zhao Wei Zhang Guo-qiang Sun 

机构地区：[1]Department of Allergy, Peking Union Medical College Hospital,Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100730, China [2]Center for Information, Peking Union Medical College Hospital,Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Beijing 100730, China

出　　处：《Chinese Medical Sciences Journal》2009年第2期86-90,共5页中国医学科学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China (30671943);the National Scientific Supporting Foundation of China (2008BAI59B04);the National High Technology Research and Development Program of China (863 Program) (2003AA2Z3502)

摘　　要：Objective To evaluate the significance of several Dermatophagoides pteronyssinus allergen extracts for skin prick test(SPT) in patients allergic to Dermatophagoides pteronyssinus.Methods Two hundred and nineteen patients enrolled in Peking Union Medical College Hospital underwent SPT and serum specific IgE assay to detect the Dermatophagoides pteronyssinus allergen.Three kinds of house dust mite allergen extracts were used for SPT,including the Dermatophagoides pteronyssinus extract prepared by our laboratory(group A),standardized Dermatophagoides pteronyssinus extract(group B),and mixed extracts of Dermatophagoides pteronyssinus and Dermatophagoides farinae(group C).Human serum specific IgE result was regarded as the reference standard for diagnosis of Dermatophagoides pteronyssinus allergy.The receiver operating characteristic(ROC) curve was used to evaluate the diagnostic performance of SPT with the extracts of three groups.Results SPT results showed that the median wheal diameter of group A,group B,and group C was 0.43,0.35,and 0.28 cm,respectively,with significant difference among three groups(P<0.05).The difference was significant between group A and B(P<0.01) as well as group A and C(P<0.01),but not between group B and C(P>0.05).There was no local urticaria or systemic allergic reactions following the procedure of SPT.Local reaction was observed in 5 patients and delayed reaction was in 2 patients of group A.As for group B and C,local reaction occurred in 3 cases and delayed reaction in 2 cases in each group.The area under ROC curve of SPT with extract in group A,group B,and group C was 0.765,0.801,and 0.782,respectively.Based on the detection results of serum specific IgE,the sensitivity of SPT in diagnosis of Dermato-phagoides pteronyssinus allergy with extract of group A,group B,and group C was 92.4%,87.0%,and 81.5%,and the specificity was 60.6%,73.2%,and 74.8%,respectively.Conclusion The Dermatophagoides pteronyssinus extract for SPT prepared by our laboratory offers good sensitivity and specificity co

关 键 词：Dermatophagoides pteronyssinus ALLERGEN skin prick test receiver operatingcharacteristic curve 

分 类 号：R446.112[医药卫生—诊断学] R751.05[医药卫生—临床医学]