镍钛形状记忆合金表面改性后与成骨细胞的生物相容性(英文)  

Biocompatibility of surface modified Nickel-Titanium shape memory alloy with osteoblasts cultured in vitro

作　　者：李强[1] 辛林伟[1] 夏亚一[2] 张钦[2] 刘文忠[2] 吴萌[2] 

机构地区：[1]桂林医学院附属医院骨科,广西壮族自治区桂林市541001 [2]兰州大学第二医院骨科研究所,甘肃省兰州市730030

出　　处：《中国组织工程研究与临床康复》2009年第25期4981-4984,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:尽管记忆合金在现代医学领域有着很广泛的应用(如骨科、牙科等),但不仅人的体液会对材料的稳定性造成影响,而且材料所释放的离子也对人体造成毒副作用。所以为了能够在临床更进一步使用这类记忆合金,对其进行表面改性是非常重要的。目的:比较类金刚石镀膜形状记忆合金和非镀膜记忆合金与体外培养成骨细胞的生物相容性。设计、时间及地点:对比观察,于2005-03/06在兰州大学第二医院骨科研究所完成。材料:材料为规格为6mm×7mm×0.5mm的类金刚石镀膜镍钛记忆合金和相同规格的非镀膜镍钛记忆合金各30片,由兰州大学材料学院提供。方法:将2种不同的镍钛合金分别加入12孔培养板中,将第3代成骨细胞悬液用DMEM培养基调节成10×108L-1,等体积加入培养孔中,37℃、体积分数为5%CO2培养箱中培养。主要观察指标:细胞的增殖情况;收集第1,2,3,4,5天的细胞培养液,测定其中的碱性磷酸酶活性和镍离子的含量。结果:类金刚石镀膜镍钛记忆合金组的细胞增殖率及培养液中碱性磷酸酶活性明显高于非镀膜组;培养液中镍离子含量明显低于非镀膜组(P<0.05)。结论:类金刚石镀膜记忆合金的组织相容性明显优于非镀膜记忆合金。BACKGROUND： Although shape memory alloy has been extensively used in modern medicine, including orthopaedics and dental surgery, the body fluid could influence the stability of biomaterial and some ions released by materials may cause toxic and side effect to human body. The technology for the modification of shape-memory materials is of crucial importance for clinical use of shape memory alloy. OBJECTIVE： To investigate the biocompatibility of diamond-like carbon （DLC） coated Nickel-Titanium shape memory alloy and uncoated shape memory alloy with osteoblasts cultured in vitro. DESIGN, TIME AND SETTING： A comparative observation. The study was performed at the Institute of Othopaedics of Lanzhou University from March to June 2005. MATERIALS： A total of 30 DLC-coated Nickel-Titanium shape memory alloy disks, 6 mm×7 mm×0.5 mm, and the same amount of uncoated ones of equal size were provided by College of Materials, Lanzhou University. METHODS： Rabbit osteoblast suspension of the third passage at density of 10 × 10^8/L were incubated with DLC-coated Nickel-Titanium shape memory alloy disks and uncoated shape memory alloy in 12-well culture plate in 5% CO2 at 37 ℃. MAIN OUTCOME MEASURES： Culture media were counted at 1, 2, 3, 4 and 5 days of culture to determine alkaline phosphatase （AKP） activity and nickel （Ni^2＋） concentration. RESULTS： The proliferation of osteoblasts and the concentration of AKP in DLC- coated group were greater than uncoated group while the uncoated group released more Ni^2＋ into the cells culture media than coated group （P 〈 0.05）. CONCLUSION： DLC-coated Nickel-Titanium shape memory alloy appears to have better biocompatibility with osteoblast cultured in vitro compared to uncoated ones.

关 键 词：类金刚石 镍钛记忆合金 表面改性 成骨细胞 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]