流式细胞术在胃癌腹膜转移诊断中的应用价值  

The Value of Flow Cytometry in Diagnosing Peritoneal Metastasis from Gastric Carcinoma

作　　者：赵宏伟[1] 刘宏斌[2] 韩晓鹏[2] 朱万坤[2] 苏琳[2] 王云[2] 

机构地区：[1]兰州大学 [2]兰州军区兰州总医院普外科,兰州市730050

出　　处：《中国肿瘤临床》2009年第12期661-664,共4页Chinese Journal of Clinical Oncology

基　　金：全军科学技术研究"十一五"计划基金资助(编号:06MA082)

摘　　要：目的:探讨检测胃癌腹膜转移诊断的方法。方法:收集50例胃癌患者及10例胃良性病变患者的腹腔冲洗液及临床资料,同时收集胃癌原发灶组织标本做阳性对照。采用流式细胞术检测标本中肿瘤标志物CK19,同时采用HE染色进行腹腔冲洗液细胞学(PLC)检查。结果:胃癌患者腹腔冲洗液中CK19的阳性表达率为62.0%(31例),明显高于PLC检测24.0%(12例)(P<0.01),且阳性检出率随着肿瘤浸润深度、TNM分期增加而增加。50例胃癌原发灶中CK19的阳性表达率为100%,10例良性病变患者腹腔冲洗液中CK19无阳性表达。结论:流式细胞术可作为预测胃癌腹膜种植转移的手段。其检测CK19可提高腹腔冲洗液中游离癌细胞的检测灵敏度。Objective： To explore a method for predicting peritoneal metastasis in gastric carcinoma patients. Methods： Peritoneal washings and clinical data from 50 patients with gastric carcinoma and 10 patients with benign gastric diseases were collected. We used flow cytometry to detect the cytokeratin 19 （CK19） in peritoneal washings. Peritoneal lavage cytology （PLC） examinations were carried out after H＆E staining. Results： The positive rate of CK19 in peritoneal washings from gastric carcinoma patients was 62.0% （31 cases） based on flow cytometry data. The positive rate of CK19 was significantly higher using the flow cytometry data than the PLC analysis （24.0% or 12 cases with PLC）. The positive rate of CK19 increased with the depth of tumor invasion, TNM staging and degree of peritoneum involvement. Conclusion： Flow cytometry can be used to predict peritoneal metastasis of gastric carcinoma. Detecting CK19 in peritoneal washings using flow cytometry can improve the sensitivity of free tumor cell detection.

关 键 词：流式细胞仪 胃癌 腹膜转移 CK19 

分 类 号：R735.2[医药卫生—肿瘤]