PP60C-SRC/ERK1/2信号通路调节大鼠精原干细胞活性  

PP60C-SRC/ERK1/2 regulates the viability of rat spermatogonial stem cells

作　　者：陈加祥[1] 徐斯凡[2] 王晶磊[1] 杨俊玲[1] 秦海燕[1] 邹挺[1] 

机构地区：[1]南昌大学医学院生理教研室,江西南昌330006 [2]中央民族大学生命与环境科学学院,北京100081

出　　处：《基础医学与临床》2009年第7期678-682,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30360032);南昌大学(医科类)基金(NC2007004);江西省教育厅基金(GJJ09444)

摘　　要：目的研究PP60C-SRC通过磷酸化的细胞外调节激酶1/2(p-ERK1/2)调节体外培养的9日龄大鼠精原干细胞的活性。方法用MTT观察反义C-SRC脱氧寡核苷酸(ODNs)及PD98059对精原干细胞活性的影响;用W est-ern b lot检测PP60C-SRC和p-ERK1/2的表达。结果与空白对照组相比,10μmol/L反义C-SRCODNs作用12 h后精原干细胞活性下降了8.1%(P<0.05),10μmol/L PD98059作用24 h后精原干细胞活性下降了9.4%(P<0.01)。反义C-SRCODNs组PP60C-SRC、p-ERK1/2蛋白表达与空白对照组相比分别下降了33.8%和45.3%(均P<0.01);10μmol/L PD98059作用精原干细胞24 h后,p-ERK1/2蛋白表达下降了34.5%。结论PP60C-SRC可能通过p-ERK1/2蛋白而调节精原干细胞的活性。Objective To investigate the effect of PP60C-SRC on the viability of rat spermatogonial stem cells in 9 day-old rat. Methods MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense C-SRC oligodeoxynucleotides （ODNs） and PD98059 in vitro; Western blot was used to observe the PP60C-SRC and phosphorylated extracellular regulated kinase1/2 （p-ERK1/2） contents. Results Compared with that in control group, the viability of the cells was decreased by 8.1% （P 〈 0. 05 ） and 9. 4% （ P 〈 0. 01 ）, respectively, after treatment with antisense C-SRC ODNs and PD98059. Compared with the control group, PPt0C-SRC and p-ERK1/2 contents were decreased by 33.8% and 38.4% （ both P 〈 0. 01 ）, respectively, after transfection of antisense C-SRC ODNs. Compared with the control group, the p-ERK1/2 content was decreased by 34. 5% （P 〈 0. 01 ） in the spermatogonial stem cells after treatment with PD98059. Conclusion PP60C-SRC regulates the viability of rat spermatogonia stem cells through p-ERK1/2.

关 键 词：精原干细胞 PP60C—SRC 细胞外调节激酶1/2 大鼠 

分 类 号：R363[医药卫生—病理学]