新型的动物模型-可示踪胰岛β细胞发育的转基因斑马鱼  

A new model-tracking of β-cell development by generation of transgenic zebrafish

作　　者：夏铭[1] 潘雪[1] 邓敏 金怡[3] 陈漪[3] 王和生[1] 孔德明[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]上海健康科学研究所 [3]上海瑞金医院血液学研究所,上海200025

出　　处：《基础医学与临床》2009年第7期721-725,共5页Basic and Clinical Medicine

基　　金：省长课题基金(黔省专合字(2006)109号);贵州省中药现代化科技产业研究开发专项(黔科合中药专字[2007]5007号)

摘　　要：目的建立活体可视化胰岛β细胞发育的立体表达动物模型。方法利用分子克隆、显微注射、全胚胎原位杂交和活体摄像等方法,建立绿色荧光蛋白标记胰岛β细胞的转基因斑马鱼。结果建立并筛选获得胰岛β细胞发育的转基因斑马鱼,证实荧光蛋白与内源性胰岛素的共定位表达,实现活体动态监测胰岛β细胞的发育情况。结论胰岛β细胞的转基因斑马鱼动物模型实现在整体动物水平上研究胰岛β细胞的发育。Objective To establish a pancreatic β-cell developmet fish model with specific spatial expression patterns. Methods Molecular cloning, microinjection, whole embryo in-situ hybridization （WISH） and fluorescence microscopy in living were used to analyze of β-cell development through generation of transgenic zebrafish. Results Screened and established pancreatic β cells of transgenic zebrafish, and confirmed the fluorescence protein expression in the same spatiotemporal pattern with endogenous insulin gene to achieve dynamic monitoring islet β-cell development situation in vivo. Conclusion The pancreatic β cells of transgenic zebrafish animal model can successfully trace pancreatic 13 cell development.

关 键 词：斑马鱼 胰岛Β细胞 转基因 胰岛素 荧光蛋白 

分 类 号：R-332[医药卫生]