人骨形态发生蛋白3抑制骨肉瘤细胞系MG63和U2OS的体外生长  

Human bone morphogenetic protein 3(hBMP3) inhibits growth of human osteosarcoma cells MG63 and U2OS

作　　者：吴丽美[1] 何焕玲[1] 李星星[1] 陈英华[1] 卫佳[1] 左国伟[1] 苗静琨[1] 王嫣[1] 周兰[1] 

机构地区：[1]重庆医科大学生物医学工程系重庆市生物医学工程学重点实验室重庆市超声医学工程重点实验室-省部共建国家重点实验室培育基地,重庆400016

出　　处：《基础医学与临床》2009年第7期731-736,共6页Basic and Clinical Medicine

基　　金：重庆市自然科学基金(CSCT2005BB5320)

摘　　要：目的研究人骨形态发生蛋白3(hBMP3)对骨肉瘤细胞系MG63和U2OS的生物学作用。方法hBMP3的重组腺病毒和表达hBMP3的条件培养液(rhBMP3-CM)干预MG63和U2OS,台盼蓝拒染法、TUNEL法、吖啶橙/溴乙啶荧光双染法、Transwell小室和碱性磷酸酶活性测定法分别检测细胞增殖、凋亡、迁移以及成骨分化能力的变化;空白组不做任何处理,实验对照组用AdGFP和rGFP-CM处理。结果实验对照组和空白组间差别无显著性。与各自的对照组相比,在观察时间内,两种处理均致实验组细胞:(1)存活率降低;(2)凋亡率增加;(3)穿膜细胞数减少;(4)碱性磷酸酶活性增加。结论hBMP3对骨肉瘤细胞系具有抑制作用,并促其向成骨方向分化。Objective The biological effects of BMP3 on osteosarcoma were investigated by treating human osteosarcoma cell lines MG63, and U2OS with human BMP3 （hBMP3）. Methods Osteosarcoma cells in experimental groups were respectively treated with AdBMP-3 and rhBMP3-CM, control groups with AdGFP and rGFP-CM, the blank group with neither. Their ability of proliferation, apoptosis, transmigration and differentiation were respectively detected by trypan blue exclusion test, terminal deoynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） and acridine orange-ethidium bromide fluorescent stain （AO/EB）, transwell-room and alkaline phosphatase（ALP） activity reagent kit. Results （1） All the indexes detected were not significantly different between two control groups. （2） Compared with control groups, the cell survival rate showed a significant decrease in experimental groups. （3） The apoptosis indexes increased. （4）The number of trans-membrane cell decreased. （5） The activity of alkaline phosphatase increased after treatment with AdBMP3 and rhBMP-3 for 3 days in MG63,5 days in U2OS. Conclusion hBMP3 inhibit osteosareoma cells growth and promote bone formation.

关 键 词：骨形态发生蛋白 骨肉瘤 细胞增殖 细胞凋亡 细胞迁移 

分 类 号：R738.1[医药卫生—肿瘤]