少突胶质细胞瘤染色体1p／19q杂合性缺失与p53蛋白表达的相关性研究  

Loss of heterozygosity of chromosome 1p/19q and pS3 protein expression in oligodendroglioma

作　　者：熊佶[1,2] 刘颖[1] 李超[2] 朱静静[2] 叶诸榕[1] 毛颖 汪寅[2] 

机构地区：[1]复旦大学上海医学院病理学系,200032 [2]复旦大学附属华山医院神经病理室 [3]华山医院神经外科

出　　处：《中华病理学杂志》2009年第7期445-450,共6页Chinese Journal of Pathology

基　　金：复旦大学附属华山医院科研启动基金

摘　　要：目的探讨少突胶质细胞瘤染色体10／19q杂合性缺失及p53蛋白的表达情况，与星形细胞起源的肿瘤进行比较研究并探讨其意义。方法选择2004—2005年间，经病理组织学诊断为不同类型和级别的胶质瘤合计191例，包括：WHO1I级少突胶质细胞瘤116例，其中30例为新鲜组织；间变性少突胶质细胞瘤45例和不同级别星形细胞起源的肿瘤石蜡组织30例；采用PCR-微卫星技术检测染色体1p／19q杂合性缺失情况；采用免疫组织化学方法对184例胶质瘤石蜡切片p53蛋白表达情况进行半定量分析。结果86例WHOⅡ级少突胶质细胞瘤石蜡标本染色体1p缺失率为69．8％（60／86）、19q缺失率为64．0％（55／86）、1p／19q联合缺失率为57．0％（49／86）；45例间变性少突胶质细胞瘤中，1P缺失率为71．1％（32／45）、19q缺失率为60．0％（27／45）、1p／19q联合缺失率为55．6％（25／45）；两种级别间差异无统计学意义（P〉0．05）。30例WHOⅡ级少突胶质细胞瘤新鲜标本染色体1p缺失率为70．0％（21／30）、19q缺失率为63．3％（19／30）、1p／19q联合缺失率为60．0％（18／30），与石蜡标本的缺失率比较差异无统计学意义（P〉0．05）。30例星形细胞起源的肿瘤染色体对应三种缺失率分别为23．3％（7／30）、33．3％（10／30）及20．0％（6／30），与少突胶质细胞瘤差异有统计学意义（P〈0．05）。86例WHOⅡ级少突胶质细胞瘤中，仅7例有p53蛋白阳性表达（占8．1％）；45例间变性少突胶质细胞瘤中，有14例呈阳性表达（31．1％），两者差异有统计学意义（P=0．007）。少突胶质细胞瘤053蛋白阳性表达明显低于星形细胞起源的肿瘤（P=0．001）。在间变性少突胶质细胞瘤中，染色体10／19q杂合性缺失与053蛋白阳性表达呈负相关（P〈0．05）。结论石蜡和新鲜组织均可用于染色体1p／19q杂合性缺失的检测。在Objeetive To study the status of loss of heterozygosity （LOH） of chromosome 1p/19q and p53 protein expression in oligodendroglioma, as compared to astrocytoma. Methods One hundred and ninety-one cases of glioma of different histologic types and grades, including 116 cases of low-grade of oligodendroglioma （86 paraffin-embedded and 30 fresh tissues） , 45 cases of anaplastic oligodendroglioma （al1paraffin-embedded tissues ） and 30 cases of astrocytoma of various grades （al1paraffin-embedded tissues）, were enrolled into the study. The LOH of chromosome 1p/19q was investigated by polymerase chain reaction （PCR）-based microsatellite analysis. The p53 protein expression was demonstrated by immunohistochemical staining. Results The rates of 1p loss, 19q loss and 1p/19q loss were 69. 8% ,64%, and 57.0% respectively in the 86 paraffin-embedded low-grade oligodendroglioma samples, as compared to 71.1%, 60.0% and 55.6% respectively in the 45 paraffin-embedded anaplastic oligodendroglioma samples. There was no difference of LOH of 1p/19q between low-grade oligodendroglioma and anaplastic oligodendroglioma （ P 〉 0. 05 ）. In the 30 cases of low-grade oligodendroglioma with fresh tissues available, the rates of 1p loss, 19q loss and 1p/19q loss were 70.0%, 63.3% and 60. 0% respectively. The LOH of 1p/19q between paraffin-embedded and fresh samples was not statistically significant （P 〉0. 05）. In the 30 cases of astrocytoma, the rates of 1p loss, 19q loss and 1p/19q loss were 23.3%, 33.3% and 20. 0% respectively, which were significantly less than those in oligodendroglioma （P 〈 0. 05 ）. The expression of p53 protein was significantly lower in low-grade oligodendroglioma （8. 1% ） than in anaplastie oligodendroglioma （31.1%, P = 0. 007 ）. The expression of p53 protein in oligodendroglioma was also lower than in astrocytoma （ P = 0. 001 ）. Furthermore, p53 protein expression negatively correlated with 1p/19q loss in anaplastic oligodendroglioma （P 〈 0. 05 ）. Conclusions Both

关 键 词：少突神经胶质瘤 染色体 人 1对 染色体 人 19对 杂合子丢失 肿瘤抑 制蛋白质p53 

分 类 号：R686[医药卫生—骨科学]