脾脏切除对实验性脑损伤大鼠生存率及血液、脑组织中IL-1β含量的影响  

Effects of splenectomy on mortality and IL-1β content in blood and traumatic brain tissue in rat with experimental brain trauma

作　　者：李梅[1] 吴国材[1] 李飞[1] 张礼均[1] 陈志[1] 张项[1] 单佑安[1] 林江凯[1] 朱刚[1] 尹志勇[2] 冯华[1] 

机构地区：[1]第三军医大学西南医院神经外科全军神经系统疾病微创诊治专科中心,重庆400038 [2]第三军医大学野战外科研究所,重庆400042

出　　处：《创伤外科杂志》2009年第4期302-305,共4页Journal of Traumatic Surgery

基　　金：卫生部行业公益性基金(200802017);973前期专项基金(2007CB516704)

摘　　要：目的观察脾脏切除对实验性脑损伤大鼠死亡率、血液中白细胞介素-1β(IL-1β)的浓度以及致伤区脑组织IL-1βmRNA含量的影响,为提高重型颅脑创伤患者救治水平探讨新思路。方法成年雄性SD大鼠随机分为3组:颅脑创伤假手术+脾切除假手术组(A组),颅脑创伤+脾切除假手术组(B组)和颅脑创伤+脾切除组(C组)。采用Feeney自由落体法对大鼠右顶叶致伤,观察各组大鼠致伤后7天内死亡率(nA=23,nB=65,nC=65);ELISA法测定致伤后6、12、24小时、2天及3天时大鼠血液中IL-1β的浓度(各时相点nA=nB=nC=6);荧光定量PCR法检测致伤后6、12、24小时、2天及3天时致伤区域脑组织IL-1βmRNA的含量(各时相点nA=nB=nC=3)。结果致伤后7天内大鼠A、B、C组死亡率分别为0%,38.46%及16.92%,各组间比较均具有显著差异(P<0.05);ELISA检测发现致伤后2天及3天时B组IL-1β显著增高,A、B及C组分别为:184.3±31.6、355.3±58.1(P<0.01)及140.4±29.5,109.4±16.7、1205.6±128.8(P<0.01)及165.0±20.1;荧光定量PCR检测发现,B、C两组致伤侧脑组织IL-1βmRNA含量均出现先显著升高、后下降的趋势,但C组较B组下降明显。结论颅脑创伤大鼠于伤后行脾脏切除能够显著降低大鼠死亡率,对血液及创伤区域脑组织中IL-1β的产生均有一定的抑制作用。Objective To observe the effect of spleneetomy on the mortality and IL-1β concentration in blood and its mRNA content in traumatic brain tissue of rats with experimental brain trauma, so as to provide effective clin- ical management for severe traumatic brain injury patients. Methods Adult male SD rats were divided into 3 groups randomly ： sham operation on brain and spleen I group A ） , experimental brain trauma ＆ sham operation on spleen（group B） ,and experimental brain injury ＆ splenectomy（ group C）. Modified Feeney＇s method was used to create the animal model of experimental brain trauma. Mortality within 7 days following the experiment was calculat- ed （ nA = 23, nB = 65, nc = 65 ） , and IL -1β concentration in blood at 6,12,24 hours, 2 days, and 3 days after exper- iment was examined by ELISA （ nA = nB = nc = 6 ） ; fluorescent quantitation of IL-1β mRNA was also determined. Results The mortality within 7 days after experiment in group A,B,and C was 0% ,38.46% ,and 16.92% respec- tively （ P 〈 0.05, significant difference was observed between groups） ： at day 2, IL-1β concentration in group A, B, andCwas 184.3 ±31.6,355.3 ±58.1（P〈0.01）,140.4±29.5 respectively; at day 3,109.4±16.7,1205.6± 128.8 （ P 〈 0.01 ） , 165.0 ± 20.1 respectively ; IL-1βmRNA content was highly increased at 6 hours after experiment and then decreased in both group B and C. Conclusion Splenectomy after experimental brain trauma in rat could effectively reduce its mortality, and could inhibit IL-1β expression in some level.

关 键 词：颅脑损伤 脾脏切除 死亡率 白细胞介素-1Β 大鼠 

分 类 号：R651.15[医药卫生—外科学] R657.6[医药卫生—临床医学]