脑胶质瘤组织中MDM2、p53、p14^(ARF)和p21^(WAF1)蛋白表达及意义  

MDM2,p53,p14^(ARF) and p21^(WAF1) Protein Expression in Gliomas and its Significance

作　　者：曾义[1,2] 龙晓东[1] 游潮[2] 徐宏[1] 

机构地区：[1]四川德阳市人民医院,四川德阳618000 [2]四川大学华西医院,四川成都610041

出　　处：《肿瘤学杂志》2009年第6期541-544,共4页Journal of Chinese Oncology

摘　　要：[目的]探讨MDM2、p53、p14ARF和p21WAF1蛋白在胶质瘤组织中的表达及意义。[方法]应用免疫组化SP法检测胶质瘤组织中MDM2、p53、p14ARF和p21WAF1蛋白的表达情况,并分析其与胶质瘤组织学分级之间的关系。[结果]Ⅱ级和Ⅳ级胶质瘤中,MDM2阳性表达率分别为24.00%(6/25)及56.52%(13/23)(χ2=5.298,P=0.021);p53阳性表达率分别为28.00%(7/25)及60.87%(14/23)(χ2=5.259,P=0.022)。p14ARF的阳性表达率分别为76.00%(19/25)及34.78%(8/23)(χ2=8.270,P=0.004),p21WAF1的阳性表达率分别为76.00%(19/25)及39.13%(9/23)(χ2=6.700,P=0.010)。[结论]胶质瘤组织中,MDM2和p53均呈不同程度的过度表达,且随胶质瘤恶性程度的增加而表达水平增高,但p14ARFandp21WAF1蛋白随胶质瘤恶性程度的增加而表达降低。MDM2过表达、p53突变以及p14ARF、p21WAF1蛋白表达降低与胶质瘤的演进有关。[Purpose] To investigate the expressions of MDM2, p53, p14^ARF and p21^WAF1 proteins in gliomas and their biological significance. [Methods] Expressions of MDM2, p53,p14^ARF and p21^WAF1 protein were determined in glioma by immunohistochemical streptavidin-peroxidase technique. [Results] In grade Ⅱ and grade Ⅳ glioma, MDM2 positive rates were 24.00%（6/25） and 56.52%（13/ 23） （Х^2=5.298,P=0.021）; p53 positive rates, 28.00% （7/25）and 60.87% （14/23） （Х^2=5.259,P= 0.022）; and p14^ARF positive rates, 76.00%（19/25）amt 34.78%（8/23）（Х^2=8.270,P=0.004）, and p21^WAF1 positive rates, 76.00%（19/25） and 39.13%（9/23） （Х^2=6.700,P=0.010） respectively. [Conclusion] MDM2 and p53 protein are over-expressed to a certain extent in glioma and the positive rates elevate along with aggressive extent of glioma, but positive rates of p14^ARF and p21^WAF1 decrease along with the glioma aggression. Over-expression of MDM2, mutation of p53, and low-expression of p14^ARF and p21^WAF1 might be related to progression of glioma.

关 键 词：脑肿瘤 胶质瘤 MDM2 P53 P14^ARF P21^WAF1 

分 类 号：R739.41[医药卫生—肿瘤]