基于生物发光的高灵敏度ATP自动测试系统研究  

Study of an automatic ATP detecting system with high sensitivity based on bioluminescence

作　　者：岳伟伟[1,2] 何保山[1,2] 周爱玉[1,2] 罗金平[1,2] 蔡新霞[1,2] 

机构地区：[1]中国科学院电子学研究所传感技术国家联合重点实验室,北京100080 [2]中国科学院研究生院,北京100080

出　　处：《高技术通讯》2009年第6期609-614,共6页Chinese High Technology Letters

基　　金：国家863项目(项目编号No.2007AA04Z326);北京市科学技术委员会(项目编号:Z0006302040191);以及国家自然科学基金(项目编号No.60576049)对本项目的资助

摘　　要：针对高灵敏度、宽响应范围及自动检测三磷酸腺苷(ATP)的需求,设计了一种基于生物发光原理的ATP检测系统。系统采用自动加样技术,在发光反应的同时开始记录光强,使仪表检测自动化,且提高检测灵敏度;在优化光电检测单元设计和减小杂散光及电磁干扰的同时,提高荧光检测效率;通过电路和数据处理算法优化提高仪表检测范围。以浓度为10^(-15)～10^(-6)M范围内的标准ATP溶液进行了实验,结果表明仪表测量光强与ATP实际浓度相关系数为0.974,检测范围达9个数量级。与市场相关仪器相比,该系统具有自动化测试,性能和灵敏度高、响应范围宽的特点,在ATP测量以及相关领域如细菌测量、毒性测试及卫生状况检测等方面具有广泛的应用前景。To meet the needs of automatic adenosine triphosphate （ATP） detection with high sensitivity and wide response range, an ATP detection system was designed based on bioluminescence. By recording the light intensity at the beginning of the bioluminescence reaction using the automatic injection technology, the system can detect samples automatically with high sensitivity. The photo-electronic detection unit was optimized to decrease the influence of stray light and electromag- netism and increase the efficiency of luminescence detection. The circuit and data processing algorithm was optimized to broaden the detection range. The ATP samples with the concentration of 10^-15-10^-6 M were measured and the correla- tion coefficient between the ATP concentration and the light intensity was 0.974. The detection range was over 9 magni- tudes. Compared with the commercial products, the system in this paper has the virtues of automatization, high sensitivity and wide detection range. Therefore, the ATP system has a great potential in ATP measurement and the corresponding fields such as bacteria, toxicity and hygienic conditions, and so on.

关 键 词：三磷酸腺苷(ATP) 生物发光 检测系统 自动加样 

分 类 号：TP274[自动化与计算机技术—检测技术与自动化装置] Q63[自动化与计算机技术—控制科学与工程]