肉种鸡饲养方式对子代胚肝蛋白表达谱的影响  

Effect of Maternal Rearing on the Proteins Profiles of Liver in Offspring Broiler during Embryonic Development

作　　者：黄建珍[1,2] 唐雪[1] 阮记明[2] 马海田[1] 邹思湘[1] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]江西农业大学动物科技学院,南昌330045

出　　处：《畜牧兽医学报》2009年第6期855-861,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展规划项目(2004CB117505);(973计划项目)国家自然科学基金项目(30600439)资助

摘　　要：挑选40周龄体况良好的产蛋三黄肉种鸡200只,随机分为对照组(笼养)和试验组(平养),饲喂相同的肉种鸡日粮。收集受精种蛋各300枚并孵化,分别取9、14、19胚龄和出壳时的肝脏,经2D-PAGE分离后,对经胶体考马斯亮蓝G-250染色的图像用PDquest7.3软件进行差异表达分析,取差异表达的蛋白质斑点进行胶内酶切和MALDI-TOF-MS分析。结果表明,试验组和对照组在9、14和19胚龄时,胚质量和肝质量均无显著差异;出壳时,试验组胚质量、肝质量均显著小于对照组(P<0.05)。蛋白表达谱分析发现29个在试验组和对照组间表达量存在两倍以上差异的蛋白斑点,其中22个得到鉴定。在这些被鉴定的蛋白中,大多涉及到机体内基础代谢的酶、能量产生及信号传导等。Forty-week healthy Sanhuang （SH） broilers were randomly divided into floor-reared （treatment） and cage-reared groups （control）, and feed with the same diet. During the experi- mental period, 300 fertile eggs were randomly collected from each group and directly incubated in an electric forced-draft incubator. Livers were collected on day-9, 14, 19 of incubation and the hatching, respectively. Proteins were extracted and separated by 2D-PAGE and stained with col- loidal Coomassie Brilliant Blue. Then, the stained gels were scanned and analyzed using PDQuest7.3 software. The differential protein spots with in-gel trypsin digestion were subse- quently identified by MALDI-TOF-MS. The results indicated that the embryo and absolute liver weight of floor-reared group was significantly lower than that of the control at hatching, although no significant differences were observed in both embryo and absolute liver weight between the floor-reared group and the control on day 9, 14 and 19 of embryonic development. The result of offspring broiler liver protein profiles showed that twenty-nine proteins had expressed at least 2.0-fold variance compared to the control during embryonic development and twenty-two of them were identified. Most of these proteins were highly related to carbohydrate metabolism, energy production and signal transduction.

关 键 词：肉鸡 饲养方式 2D-PAGE 

分 类 号：S831.4[农业科学—畜牧学]