黄连素抑制促炎症因子生成保护多巴胺能神经元  

Inhibitory effect of Berberine on inflammation-induced dopaminergic neuron-degeneration

作　　者：张宇红[1] 汪锡金[2] 叶民[2] 陈玉娟[1] 杨红彦[1] 刘学源[1] 

机构地区：[1]同济大学附属第十人民医院神经内科,上海200072 [2]上海交通大学附属瑞金医院神经内科,上海200025

出　　处：《同济大学学报（医学版）》2009年第3期42-45,64,共5页Journal of Tongji University(Medical Science)

基　　金：上海市博士后基金资助项目(05R214125);中国博士后基金资助项目(2005038433);上海市科委重点资助项目(08411950900);上海市科委重点资助项目(044119634)

摘　　要：目的探讨黄连素对炎症介导的多巴胺能神经元损伤的影响。方法于中脑原代神经元-胶质细胞培养体系中，应用脂多糖建立多巴胺能神经元损伤的炎症机制模型，黄连素（10、20μg/ml和40μg/ml）与脂多糖（1-100 ng/ml）同时处理该培养体系。免疫细胞化学法检测酪氨酸羟化酶阳性神经元的数目，液闪测定法测定多巴胺摄取力，并检测促炎症因子，包括肿瘤坏死因子α（TNF-α）、白介素-1β（IL-1β）（ELISA法）、一氧化氮（NO）（Griess反应）和超氧化物（细胞色素c还原法）的变化。结果黄连素（10、20μg/ml和40μg/ml）对脂多糖（1-100 ng/ml）诱导的中脑多巴胺能神经元损伤具显著的保护作用，且呈剂量依赖性；黄连素也显著降低促炎症因子TNF-α、IL-1β、NO和超氧化物的生成。结论黄连素抑制促炎症因子生成，保护多巴胺能神经元。Objective To evaluate the inhibitory effect of Berberine on inflammation induced dopaminergic neuro-degeneration. Methods A model of inflammation-induced dopaminergic neuro-degeneration was established by lipopolysaccharide stimulus in mesencephalic neuron glia. Both Berberine （ 10, 20 and 40 μg/ml） and lipopolysaccharides （ 1 - 100 ng/ml） were administrated into the culture system. The number of tyrosine hydroxylase positive cells was determined by immunocytochemistry. Cell bioactivity was assessed by [3^H] DA uptake analysis. Changes of inflammatory factors such as TNF-α, IL-1β （by ELISA）, NO （by Griess method） and superoxide （by cytochrome C method） were examined. Results Berberine （10, 20 and 40 μg/ml ） dose-dependently protected mesencephalic dopaminergic neuron from degeneration induced by lipopolysaccharide （1 - 100 ng/ml ）. Besides, Berberine inhibited significantly the production of TNF-α, IL-1β, NO and superoxide significantly. Conclusion Berberine may protect mesencephalic dopaminergic neurons by inhibiting the production of proinflammatory factors.

关 键 词：黄连素 多巴胺能神经元 炎症 

分 类 号：R741.01[医药卫生—神经病学与精神病学]