登革病毒C基因突变对病毒RNA复制的影响  

Effect of mutations in dengue virus C gene on viral RNA replication

作　　者：刘忠钰[1] 于学东[1] 赵慧[1] 刘然[1] 尉雁[1] 李晓峰[1] 邓永强[1] 姜涛[1] 于曼[1] 秦成峰[1] 秦鄂德[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《军事医学科学院院刊》2009年第3期216-219,223,共5页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的:分析C基因及其编码蛋白突变对登革病毒RNA复制的影响。方法:利用融合PCR方法在登革病毒复制子中引入C基因相应部位的缺失突变,将复制子RNA转染细胞后,利用报告基因活性分析、间接免疫荧光及实时定量PCR等方法分析其复制水平。结果:获得了含有不同α螺旋缺失突变的C基因的复制子质粒,并发现Δα1复制子复制水平明显下降。结论:登革病毒C蛋白α1螺旋或其编码序列对病毒RNA复制具有重要作用。Objective：To analyze the effect of mutations in dengue virus C gene and its encoding protein on viral RNA replication. Methods：Deletions corresponding to different parts of C gene were introduced into dengue virus replicon by overlapping-PCR, the replicon RNA was transfected into BHK21 cells and replication was analyzed by reporter activity assay, immunofluorescence assay and quantitative RT-PCR. Results：Replicon plasmids with different a-helix deletions of C gene were constructed, and △α1 replicon showed reduced replication compared with wild type replicon. Conclusion： The α1-helix of dengue virus C protein or its coding sequence in C gene plays an important role in viral RNA replication.

关 键 词：登革病毒 C基因 复制子 RNA复制 

分 类 号：R373.33[医药卫生—病原生物学]