人卵巢癌靶向超声造影剂的制备及其体外寻靶能力观察  

Preparation of targeted liposome microbubbles and observation of its targeted ability in human ovarian cancer in vitro

作　　者：贺娟[1] 孙江川[1] 常淑芳[1] 李潘[2] 王志刚[2] 

机构地区：[1]重庆医科大学附属第二医院妇产科,重庆400010 [2]重庆医科大学超声影像研究所,重庆400010

出　　处：《中国医学影像技术》2009年第6期929-931,共3页Chinese Journal of Medical Imaging Technology

基　　金：863科技攻关项目(2006AA02Z4F0);国家自然科学基金(3D8D1228);重庆市卫生局项目(07-2-098)

摘　　要：目的制备人卵巢癌细胞靶向超声造影剂(TUCA),对其进行鉴定,观察其体外寻靶能力。方法采用共价结合法制备TUCA即黄体生成素释放激素-脂质体微泡(LHRH-LM):免疫荧光染色试验证明配体LHRH与LM的结合,倒置显微镜下观察TUCA与人卵巢癌细胞株OVCAR-3的结合情况,观察LHRH-LM对OVCAR-3细胞的体外寻靶能力;同时以LM为对照,并用LHRH做阻断试验。结果LHRH-LM的荧光免疫染色试验阳性;体外寻靶试验显示携带配体LHRH的LM能较好的黏附在OVCAR-3细胞上,经反复洗涤不掉,而作为对照的LM与OVCAR-3细胞株混合反应后经反复洗涤,微泡与细胞完全分离,无结合;LHRH成功阻断TUCA与癌细胞的结合。结论采用共价结合法成功制备了靶向超声造影剂,该造影剂在体外能高效地与人卵巢癌细胞株OVCAR-3结合。Objective To prepare human ovarian cancer--targeted liposome microbubbles, and to assess its targeted ability in vitro. Methods Ligand LHRH was attached to the surface of self-made liposome microbubbles with covalent bonding to prepare targeted microbubbles. Immunofluorescent staining assay was used to identify the combination of LHRH with liposome microbubbles; the targeted microbubbles were added to ovarian cancer cells OVCAR-3 and then were observed under the light and fluorescence microscope to evaluate the targeting ability of the targeted liposome microbubbles with ovarian cancer cells in vitro. At the same time, LHRH was used to perform a block experiment compared with the common liposome microbubbles. Results Targeted microbubbles were positive in immunofluorescent straining assay. In vitro, study of the targeting ability showed the targeted microbubbles could actively adhere to OVCAR-3 cell, while the control was negative. And LHRH could effectively block the targeted microbubbles. Conclusion The targeted liposome microbubbles can be pre- pared successfully with covalent bonding method and effectively bound to human ovarian cancer cells specially in vitro.

关 键 词：靶向脂质微泡 卵巢肿瘤 黄体生成素释放激素 

分 类 号：R737.31[医药卫生—肿瘤] R3[医药卫生—临床医学]