高温灭菌工艺对纳米银颗粒分散效果的影响  

Influence of high temperature sterilization on dispersing of silver nanoparticles

作　　者：汤京龙[1,2] 奚廷斐[1,2] 刘国权[1] 熊玲[2] 王硕[2] 王建宇[2] 刘丽[2] 李佳戈[2] 苑富强[2] 

机构地区：[1]北京科技大学材料科学与工程学院,北京100083 [2]中国药品生物制品检定所医疗器械监督检验中心,北京100050

出　　处：《材料热处理学报》2009年第3期6-9,共4页Transactions of Materials and Heat Treatment

基　　金：国家自然科学基金(30770579);国家“973”项目(2007C13936101);国家“863”项目(2007AA021901)

摘　　要：探讨了高温灭菌工艺(121℃,110.35kPa)对纳米银颗粒在两种模拟体液(PBS溶液和DMEM培养液)中的分散状况的影响,并用TEM观测纳米银颗粒在两种溶液中的尺寸和分散程度及用XPS检测溶液中的纳米银颗粒的表面元素构成。结果表明,经高温灭菌处理后,纳米银颗粒在PBS溶液中的团聚程度明显增加;但由于纳米银颗粒表面在DMEM培养液中会发生蛋白质和氨基酸吸附,形成覆蛋白膜的纳米银颗粒,因此经高温灭菌处理后,纳米银颗粒依然能够在DMEM培养液中以单颗粒形式均匀存在。这一结果说明在DMEM培养液中的纳米银颗粒即使经过高温灭菌工艺处理,依然可以保持很好的分散状态,因此DMEM是适合于纳米银颗粒下一步临床应用的分散剂。The present study was designed to investigate the dispersing of silver nanoparticles （SNPs） in two simulated body fluids （PBS and DMEM） after high temperature sterilization（121℃,110.35kPa）. The size and state of silver nanoparticles in fluid were observed by TEM, and the constitution of silver nanoparticles surface in two simulated body fluid were measured by XPS, respectively. Results indicate that silver nanoparticles is more aggregated in PBS after high temperature sterilization. But because of the absorption of protein and aminophenol on the surface of silver nanoparticles in DMEM, the silver nanoparticles can disperse in the solution even after high temperature sterilization. Therefore, DMEM is an appropriate dispersant for silver nanoparticles.

关 键 词：纳米银颗粒 模拟体液 分散剂 

分 类 号：TG146.3[一般工业技术—材料科学与工程]