携带mda-7/IL-24的双调控溶瘤腺病毒和丝裂霉素联合治疗癌症的研究  

Study of the Anti-Tumor Efficacy by Dual Regulated Oncolytic Adenovirus Mediate Mda-7/IL-24 Combined with MMC

作　　者：张磊[1] 骆菁菁[1] 庄强[1] 田雪君[1] 钱程[1] 刘立[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2009年第4期571-577,共7页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：863项目(2006AA02Z126);973计划(2004CB518804);浙江省科技厅重点项目(2006C23006)

摘　　要：研究端粒酶逆转录酶启动子和缺失24bp双调控的溶瘤腺病毒联合化疗药物对人肺癌、宫颈癌的体外杀伤作用。采用流式细胞术检测化疗药物对病毒感染宫颈癌细胞株Hela、肺癌细胞株H460的影响;采用MTT法检测病毒联合化疗对两种肿瘤细胞以及人正常细胞株L02增殖的抑制作用;利用Hoechst 33342染色对病毒联合化疗疗法诱导的肿瘤细胞凋亡进行形态学观察;用实时定量PCR分析丝裂霉素对腺病毒增殖能力的影响。结果表明AdCN205-IL-24或携带报告基因的AdCN205-EGFP联合适当剂量的丝裂霉素后,其对Hela、H460的杀伤作用显著提高(P<0.05),相同条件下对L02无明显抑制作用,并且其复制能力不因丝裂霉素的存在而发生明显变化。To study the combination effects of oncolytic adenovirus （ double regulated by hTERT promoter and/△24） and chemotherapy against human cancer cell lines Hela,H460 in vitro. Methods： （1） To obtain the infective efficiency by the adenovirus in the presence of MMC or not; （2） to test the growth inhibition effects of combinational therapy on cancer cell lines and human normal cell line L02 by MTT assay; （3） after Hoechst 33342 dying, the apoptosis of Hela and H460 induced by the virochemotherapy is observed under fluorescent microscope; （4） viral replication ability under suitable concentration MMC is detected by Real-time PCR. The anti-tumor cell lines Hela,H460 effects of either AdCN205-IL-24 or AdCN205-EGFP are significantly improved when in combination of suitable does MMC （ P〈0. 05）, while the toxicity against normal cell line L02 is not apparent. The authors also detect no change of viral replication ability whether combined with MMC or not.

关 键 词：肿瘤特异性增殖腺病毒 肿瘤治疗 化疗 联合抑癌 实时定量PCR 

分 类 号：R73-362[医药卫生—肿瘤]