Features of intervertebral disc degeneration in rat's aging process  

作　　者：Yin-gang ZHANG Zheng-ming SUN Jiang-tao LIU Shi-jie WANG Feng-ling REN Xiong GUO 

机构地区：[1]Department of Orthopaedics, the First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061, China [2]Key Laboratory of Environment and Genes Related Diseases of Ministry of Education, Xi 'an Jiaotong University, Xi 'an 710061, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2009年第7期522-527,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No. 30400163) supported by the National Natural Science Foundation of China

摘　　要：Objective:The age-related change is important part of degenerative disc disease. However,no appropriate animal model or objective evaluation index is available. This study aimed to investigate the features of intervertebral disc degeneration in aging process of rats. Methods:22-month-old Sprague-Dawley(SD) rats were used as spontaneously occurring intervertebral disc degeneration models and 6-month-old rats as young controls. Expression of collagen types II and X was measured by immunohistochemistry. Degenerations of intervertebral discs were scored according to Miyamoto's method. Numbers and areas of afferent vascular buds were measured. The thicknesses of non-calcified and calcified layers were measured and statistically analyzed. Results:There were less collagen type II expression and more collagen type X expression in the calcified layer of the cartilage endplates and nucleus pulposus in the rats of the aged group than in the young control. There were fewer and smaller afferent vascular buds in the rats of the aged group than in the young control group. The ratio of the non-calcified to the calcified layers in the rats of the aged group significantly decreased,compared with that of the young control group(P<0.01) . Conclusion:Rats can spontaneously establish intervertebral disc age-related degeneration. The expression of collagen types II and X,numbers and areas of afferent vascular buds,the ratio of the non-calcified to the calcified layers,and water and glycosaminoglycan contents in the nucleus pulposus are sensitive indexes of intervertebral disc degeneration.Objective： The age-related change is important part of degenerative disc disease. However, no appropriate animal model or objective evaluation index is available. This study aimed to investigate the features of intervertebral disc degeneration in aging process of rats. Methods： 22-month-old Sprague-Dawley （SD） rats were used as spontaneously occurring intervertebral disc degeneration models and 6-month-old rats as young controls. Expression of collagen types II and X was measured by immuno-histochemistry. Degenerations ofintervertebral discs were scored according to Miyamoto＇s method. Numbers and areas of afferent vascular buds were measured. The thicknesses of non-calcified and calcified layers were measured and statistically analyzed. Results： There were less collagen type II expression and more collagen type X expression in the calcified layer of the cartilage endplates and nucleus pulposus in the rats of the aged group than in the young control. There were fewer and smaller afferent vascular buds in the rats of the aged group than in the young control group. The ratio of the non-calcified to the calcified layers in the rats of the aged group significantly decreased, compared with that of the young control group （P〈0.01）. Conclusion： Rats can spontaneously establish intervertebral disc age-related degeneration. The expression of collagen types II and X, numbers and areas of afferent vascular buds, the ratio of the non-calcified to the calcified layers, and water and glycosaminoglycan contents in the nucleus pulposus are sensitive indexes of intervertebral disc degeneration.

关 键 词：Intervertebral disc DEGENERATION Afferent vascular bud Calcified layer Aged 

分 类 号：S511[农业科学—作物学] S858.292