中国部分地区传染性法氏囊病病毒分子流病学调查  

Investigation on Molecular Epidemiology of Infectious Bursal Disease in Parts of China

作　　者：宇文延青[1,2] 高玉龙[1] 高宏雷[1] 祁小乐[1] 杨金雨[1] 王晓燕[1] 秦立廷[1] 林欢[1] 李俊山[1] 刘伟[1] 李铁强[1] 邓小芸[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [2]河南师范大学,河南新乡453007

出　　处：《中国家禽》2009年第12期11-14,共4页China Poultry

基　　金：国家肉鸡产业体系(nycytx-42-G3-01);973基金资助项目(2005CB523202)

摘　　要：为调查我国鸡传染性法氏囊病病毒(IBDV)的流行情况,从我国11个省(市、自治区)采集法氏囊病料,分离鉴定鸡传染性法氏囊病病毒20株,运用RT-PCR方法对分离株的VP2基因进行扩增、测序和序列分析。研究表明,20株分离毒株中有19株具有IBDV超强毒株的分子特征,即222A、256I、294I和299S,与我国IBDV超强毒Gx株的核苷酸同源率在96.7%～99.4%,推导氨基酸同源率在98.2%～100%。遗传进化分析表明,所有分离毒株具有共同的祖先。In this study,complete VP2 genes of 20 IBDV isolats from 11 provinces （city or antomous region）were amplified using RT-PCR and sequenced to determine IBDV molecular epidemiology. The results showed that a majority （19/20） of the isolates had very virulent IBDV （vvlBDV）conserved amino acids ：222A,2421,2561,294I and 299S,and only SH-h had P222. Compared to Chinese vvlBDV Gx strain,the homologous rates of nucleotide and amino atid were 97.5% to 99.4% and 98.9% to 100% ,respectively. Using serotype Ⅱ of IBDV as the outgroup, a phylogenetic tree based on VP2 nucleotide sequence was constructed. Our findings demonstrated that all isolates had a similar orgion with Gx strain and the other vvlBDV strains.

关 键 词：传染性法氏病病毒 分子流行病学 超强毒株 

分 类 号：S852.659.4[农业科学—基础兽医学] TB752.27[农业科学—兽医学]