pH梯度萃取-碱性氧化铝柱层析分离长春花中的文多灵和长春质碱  

Separation of vindoline and catharanthine from catharanthus roseus by pH gradient extraction and alkaline aluminum oxide column chromatography

作　　者：张琳[1,2] 杨磊[1,2] 祖元刚[1,2] 田浩[1,2] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学林业生物制剂教育部工程研究中心(筹),黑龙江哈尔滨15004

出　　处：《化工进展》2009年第7期1282-1286,共5页Chemical Industry and Engineering Progress

基　　金：国家"十一五"科技支撑项目(2006BAD18B0401);东北林业大学青年科研基金项目(07041)

摘　　要：利用生物碱在不同pH值下存在形式不同的性质,采用pH梯度萃取法将长春花总生物碱分为单吲哚生物碱部分(含有文多灵和长春质碱)和双吲哚生物碱部分(含有长春碱),然后采用碱性氧化铝柱层析对单吲哚生物碱部分进行分离。pH梯度萃取后得到的单吲哚生物碱部分中文多灵和长春质碱的含量分别为18.12%和11.44%,收率分别为80.86%和88.91%;经碱性氧化铝柱层析分离后,得到文多灵和长春质碱含量分别为85.56%和76.73%,收率分别为85.23%和86.34%;重结晶后文多灵和长春质碱纯度分别达到95.22%和98.46%,收率分别为92.15%和98.24%。此方法适合文多灵和长春质碱的工业化大规模生产。Single indole alkaloids（containing vindoline and catharanthine） and double indole alkaloids （containing vinblastine） were separated from the total alkaloids of Catharanthus roseus by pH gradient extraction according to their different existing statuses under different pH values. The single indole alkaloids part was further isolated by alkaline aluminum oxide column chromatography. After the process of pH gradient extraction, the contents of vindoline and catharanthine are 18.12% and 11.44%, respectively, and their recoveries are 80.86% and 88.91%, respectively. After alkaline aluminum oxide column chromatography, the contents of vindoline and catharanthine are 85.56% and 76.73%, respectively, and their recoveries are 85.23 % and 86.34%, respectively. After recrystallization, the contents of vindoline and catharanthine are 95.22% and 98.46%, and their recoveries are 92.15% and 98.24%, respectively. This process is suitable for large-scale industrial production of vindoline and catharanthine.

关 键 词：pH梯度萃取 碱性氧化铝柱层析 长春花 文多灵 长春质碱 

分 类 号：TQ028.3[化学工程]