Expression Profile Changes of Genes Involved in Lipid Metabolism Pathway During Liver Regeneration in Mice  

小鼠肝再生过程中脂质代谢相关通路中基因的表达谱变化(英文)

作　　者：袁运生[1,2] 张夕原[1,3] 严德珺[1,3] 杨婷旭[1,3] 郜尽[1,2] 俞雁[1,2] 

机构地区：[1]上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海200240 [2]上海交通大学农业与生物学院动物科学系,上海200240 [3]上海交通大学农业与生物学院食品科学与工程系,上海200240

出　　处：《Agricultural Science & Technology》2009年第2期41-45,共5页农业科学与技术（英文版）

基　　金：国家科技部重大攻关课题(2004BM711A19)资助~~

摘　　要：[ Objective ] The aim of the research was to study the expression profile changes of genes involved in lipid metabolism pathway during liver regeneration in mice. [ Method] The CCI4 induced mouse model of liver regeneration was established and the total RNA was isolated from liver tissue of mouse. Then the changes of genes involved in lipid metabolism pathway during different stages of liver regeneration were detected through micro-array chip gene technique and their specific functions were also analyzed. [ Result] Dudng the process of liver regeneration, the expression level of 98 genes involved in lipid metabolism pathway changed, which were divided into eight groups according to change trend. In the mass, the expression of genes was inhibited in the early stage and up-regulated in the late phase. And the gene expression associated with fatty acid synthesis pathway was mainly up-regulated while the catabolic pathway did not change significantly. Most of genes involved in bile acid synthesis pathway were suppressed before 4.5 d and up-regulated after 4.5 d or 7 d. [ Conclusion] During the process of liver regeneration, the genes associated with lipid metabolism are expressed in different trends, and this data should provide a specific range of genes for further studying the regulation effect of lipid metabolism related pathway on liver regeneration.1材料与方法1．1材料 试验动物：9周龄的雄性C57BIV6小鼠，自由采食和饮水，每天光照12h，黑暗12h。试剂：TRIzol试剂（Invitrogen）；RNeasy Total RNA Mini Kit（Qiagen）；基因芯片Genechip mouse genome 43020（Affymetrix）；17启动子引物序列（Affymetrix）。

关 键 词：Upid metabolism Gene expression profiles Liver regeneration Micro-array chip 

分 类 号：Q75[生物学—分子生物学]