P63及TβR-Ⅱ蛋白在食管鳞状细胞癌及其癌前病变中的表达及意义  

The Expression of P63 and TβR-Ⅱ in Human Esophageal Squamous Cell Carcinoma and Premalignant Lesions and Its Significance

作　　者：董彩红[1,2] 敖启林[1] 冯笑山[2] 郝曙光[2] 吴丽娟[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院病理研究所,同济医学院病理学系,武汉430030 [2]河南科技大学第一附属医院消化科胃镜室,洛阳471003

出　　处：《华中科技大学学报（医学版）》2009年第3期286-290,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30400192;No.30770942)

摘　　要：目的通过对食管鳞状细胞癌癌前病变及其癌组织标本中P63及转化生长因子受体Ⅱ型(TβR-Ⅱ)蛋白表达的研究,探讨两者表达与食管鳞状细胞癌的关系。方法应用免疫组织化学技术检测P63及TβR-Ⅱ蛋白在正常食管黏膜鳞状上皮和轻、中、重度非典型增生鳞状上皮以及鳞状细胞癌组织共162例中的表达。结果在食管正常鳞状上皮、非典型增生鳞状上皮及其癌组织中P63蛋白表达的阳性率呈递增趋势(P<0.01),TβR-Ⅱ蛋白表达的阳性率则呈递减趋势(P<0.01)。P63和TβR-Ⅱ蛋白的表达与性别无关(均P>0.05),但与癌组织分化程度、浸润深度和淋巴结转移均显著相关(均P<0.05)。Spearman等级相关分析结果显示P63和TβR-Ⅱ蛋白的表达与食管鳞状上皮各级病变呈负相关(rs=-0.498,P<0.05)。结论P63和TβR-Ⅱ蛋白的异常表达可能与食管鳞状细胞癌癌变及其生物学行为密切相关,联合检测二者表达可作为判断食管鳞状细胞癌发生、发展和预测转移潜能的生物学指标。Objective To investigate the expression of P63 and transforming growth factor β receptor- Ⅱ （TβR-Ⅱ ） proteins in esophageal squamous cell carcinoma （ESCC） and its premalignant lesions and the correlation of both expression with esophageal squamous cells malignant transformation processes. Methods The expression of P63 and TβR- Ⅱ proteins was detected by immunohistochemistry staining in normal squamous epithelia （NE）, mild dysplasia （MD）, moderate dysplasia （MoD）, severe dysplasia （SD） and carcinoma specimens of the esophagus which were collected from 162 cases. Results The positive expression level of P63 in esophageal carcinoma was higher than that in atypical hyperplasia tissue, with the lowest level in normal esophageal tissue （P〈0. 01）, and it was negatively related to the expression of TβR-Ⅱ in these samples （P〈0.01）. The expression of P63 and TβR-Ⅱ proteins had a positive association with pathological grading, the depth of the lesions and the lymph nodes metastasis （P〈0.05）, but there was no correlation with gender （P〉0.05）. There was a negative correlation between P63 and TβR- Ⅱ proteins expression （rs = -0. 498, P〈0.05）. Conclusion The expression of P63 and TβR- Ⅱ was possibly related to the squamous cell malignant transformation and ESCC biological behavior, and they may be the markers for evaluating the carcinogenesis, development and the metastasis potentiality of the ESCC

关 键 词：食管鳞状细胞癌 癌前病变 P63 TβR-Ⅱ 

分 类 号：R735.1[医药卫生—肿瘤]