姜黄素对人成骨肉瘤MG-63细胞增殖抑制和凋亡相关基因表达的影响  

Effects of curcumin on anti-proliferation and expression of apoptosis interrelated gene of Human Osteosarcoma MG-63 Cells

作　　者：赵振利[1] 郑燕彬[1] 陈兰英[2] 刘用金[1] 武福云[1] 李祺福[1] 

机构地区：[1]厦门大学教育部细胞生物学与肿瘤细胞工程重点实验室,福建厦门361005 [2]河南城建学院,河南平顶山467044

出　　处：《现代生物医学进展》2009年第12期2201-2205,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30871241)

摘　　要：目的:探讨姜黄素对人成骨肉瘤MG-63细胞增殖抑制及凋亡相关基因表达的影响。方法:用姜黄素处理MG-63细胞,细胞计数法检测细胞增殖抑制的效果;荧光染色观察细胞的凋亡;流式细胞仪(FCM)进行细胞周期时相分析;免疫细胞化学法和Western blotting免疫法检测细胞凋亡相关基因的表达水平。结果:随着姜黄素浓度的增加及其作用时间延长,对细胞的增殖抑制作用增强,最高抑制率可达89.07%;光镜下可观察到细胞发生染色质浓缩,细胞核凝聚和碎裂等典型的凋亡形态学改变;FCM检测结果显示细胞经姜黄素处理后出现明显的凋亡峰;免疫反应结果显示,凋亡相关基因bcl-2和P53表达水平降低,而bax和Fas表达水平升高。结论:姜黄素能显著抑制MG-63细胞增殖并可有效诱导其凋亡,其作用机制可能与bcl-2和bax二者的比值发生变化从而接受了凋亡刺激信号有关。Objective： To investigate the regulatory effect of curcumin on proliferation inhibition and apoptosis in human osterosarcoma MG-63 cells. Methods： MG-63 cells were treated by curcumin, and the inhibitory effect on cell proliferation was assessed by cell count. The apoptosis was inspected by fluorescent staining. The cell cycle analysis was detected by FCM. The expression level of genes related with apoptosis was observed by immunohistochemistry and western blotting immunoreaction. Results： The results showed that the cells growth was inhibited with the increasing of the concentration of curcumin and the elongation of the treated time,and the highest inhibition rate was 89.07%. Parts of cells presented the typical morphological changes of apoptosis： chromatin concerntrated and agglutinatied, cell nucleus shrinked and cataclased under the microscope. The results of FCM analysis showed that curcumin could induce cells into apoptosis, which was proved by the emergence of apoptotic peak. The results of immunoreaction suggested that the expression level of bcl-2 and P53 were down-regulated and the expression level ofbax and Fas were upregulated. Conclusions： Curcumin not only inhibited cell proliferation but also induced apoptosis of MG-63 cells, which may be related to the ratio of the expression level of bcl-2 and bax as well as reception of the signal of stimulating apoptosis.

关 键 词：姜黄素 人成骨肉瘤MG-63细胞 细胞凋亡 bcl-2 bax 

分 类 号：Q279[生物学—细胞生物学] R730.5[医药卫生—肿瘤]