膀胱移行细胞癌细胞中心体异常与p53基因突变关系的实验研究  

The Experimental Study on the relation of Centrosome Hyperamplification and P53 genetic mutation in Bladder Cancer

作　　者：王连渠[1] 王明[1] 谭毅[1] 

机构地区：[1]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《重庆医科大学学报》2009年第7期870-873,共4页Journal of Chongqing Medical University

摘　　要：目的:研究膀胱移行细胞癌细胞中心体异常与p53基因突变的关系,探讨中心体异常在p53基因突变导致肿瘤发生发展过程中的作用及意义。方法:采用免疫组化方法,对76例膀胱移行细胞癌标本进行中心体异常和p53蛋白表达的检测。一抗分别为鼠抗人中心体周围蛋白单克隆抗体和鼠抗人p53单克隆抗体。结果:通过对样本统计分析证实,膀胱移行细胞癌中心体异常与p53蛋白表达均是预测肿瘤预后的指标,并且两者之间呈正相关(r=0.707),具有高度统计学意义(P<0.01)。结论:中心体异常可能是p53基因突变导致膀胱移行细胞癌发生发展过程中的一个重要中间环节。研究中心体异常可能会加深对肿瘤发生机制的了解,为临床上治疗肿瘤提供新的治疗方法和思路。Objective： To explore the relation of the abnormal condition of centrosome and p53 genetic mutation of bladder cancer cells, its effect on the onset and progress of the transitional cell carcinoma of bladder because of p53 genetic mutation. Methods： 76 transitional cell carcinoma of bladder were examined. The centrosome was stained by indirect immunofluorescence methods, and the primary antibody was monoclonal antibody against pericentrin. The p53 was stained by SP immunohistochemical methods and the primary antibody was monoclonal antibody against p53 .Results： Through statistical analysis,detection of CH or p53 expression was useful in providing prognostic information in bladder cancer. There existed a positive correlated （r=0.707,P〈0.01） between the CH and the p53 genetic mutation. Conclusion：CH may be an important middle link of the onset and progress of the transitional cell carcinoma of bladder because of p53 genetic mutation. Study of CH may help to deepen understanding of the mechanism of the tumorigenesis and provide new therapy for tumor in clinic.

关 键 词：中心体异常 染色体不稳 p53 膀胱移行细胞癌 

分 类 号：R737.14[医药卫生—肿瘤]