血清粘附分子及其相关指标检测对乙肝肝硬化患者诊治的意义  

Significance of the determination on sICAM-1 and its' combined indexes in the diagnosis and treatment of liver cirrhosis

作　　者：刘仲满 

机构地区：[1]湖南省株洲化工集团医院内科,株洲412004

出　　处：《重庆医科大学学报》2009年第7期948-950,共3页Journal of Chongqing Medical University

摘　　要：目的:探讨乙肝肝硬化患者外周血清白细胞介素-8(Interleukin-8,IL-8)、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)和可溶性细胞间粘附分子-1(Soluble intercelluar adhesionmolecule-1,sICAM-1)的变化及其临床意义。方法:采用双抗体夹心酶联免疫吸附法(ELISA)和流式细胞仪技术检测41例正常人和63例乙肝肝硬化患者外周血清sICAM-1、IL-8、TNF-α水平。结果:乙肝肝硬化患者血清中sICAM-1、IL-8、TNF-α含量水平均明显高于正常对照组,且差异有统计学意义(P<0.01);失代偿期肝硬化与代偿期肝硬化比较,差异有统计学意义(P<0.05);乙肝肝硬化患者sICAM-1、IL-8增高呈正相关;乙肝肝硬化患者血清三指标含量由高到低依次为Child-PughC级、B级、A级组及正常对照组,且Child-Pugh分级各组与正常对照组比较,差异有统计学意义(P<0.01)。结论:血清sICAM-1、IL-8、TNF-α水平可反映肝硬化肝细胞损害程度和炎症活动性,对其动态观察可对病情作出有价值的评估。Objective：To investigate the change and significance of interleukin-8 （IL-8） and intercellular adhesion molecule-1 （ICAM-1） in periheral blood of 41 healthy and 63 patients with liver cirrhosis. Methods： The serum level of TNF-αand IL-8 was analyzed using enzyme-linked immunosorbent assay （ELISA）. The serum level of sICAM-1 was determined using flow cytometry. Results： Compare with normal control group,the serum level of ICAM-1 ,IL-8,and TNF-α showed significant difference （P〈0.01）, and it also showed significant difference between decompensated liver cirrhosis and the compensated liver cirrhosis. The serum level of sICAM-1 increased gradually with the Child-Pugh grade, and it was positively correlated to IL-8.Conclusion： The results show that serum levels of IL-8, sICAM-1 and TNF-α may reflect the liver damage. Dynamic observation of Serum sICAM-1 level is helpful of estimating the state of the diseases.

关 键 词：肝硬化 白细胞介素-8 可溶性细胞间粘附分子-1 肿瘤坏死因子-α 

分 类 号：R575.29[医药卫生—消化系统]