Local hypothermia and optimal temperature for stroke therapy in rats  

作　　者：WU Hao JIANG Li-dan Karsten H. Wrede JI Xun-ming ZHAO Xi-qing TIAN Xin GAO Yu-fei LING Feng 

机构地区：[1]Department of Neurosurgery Xuan Wu Hospital, Capital Medical University, Beijing 100053, China [2]Departement of Neurosurgery, International Neuroscience Institute, Rudolf Pichlmayr Str. 4, 30625 Hannover, Germany [3]Department of Neurosurgery, the People Hospital of Tangshan, Tangshan, Hebei 063000, China [4]Central Lab Xuan Wu Hospital, Capital Medical University, Beijing 100053, China [5]Department of Neurosurgery, China-Japan Union Hospital, Ji Lin University, Changchun, Jilin 130033, China

出　　处：《Chinese Medical Journal》2009年第13期1558-1563,共6页中华医学杂志（英文版）

基　　金：This research was suported by a grant from the National Natural Science Foundation of China （No. 30471780）.

摘　　要：Background Local hypothermia induced by intravascular infusion of cold saline solution effectively reduces brain damage in stroke. We further determined the optimal temperature of local hypothermia in our study. Methods Seventy-eight adult male Sprague Dawley rats （260-300 g） were randomly divided into 3 groups： group A, ischemia/reperfusion without cold saline infusion （n=26） （control group）; group B, infusion with 20℃ saline before reperfusion （n=26）; group C： infusion with 10℃ saline before reperfusion （n=26）. In each group, we chose 15 rats for monitoring physical indexes and the temperature of the brain （cortex and striatum） and body （anus）, measurement of brain infarction volume, assessment of neurological deficits and the survival rate of reperfusion at 48 hours. Another 8 rats from each group was chosen for examining brain edema, another 3 from each group for histological observation by electron microscopy （EM） and light microscopy （LM） at 48 hours after reperfusion. Results There was no significant difference among the 3 groups for physical indexes during the examination （F（2. 45）= 0.577, P=0.568; F（2.45）= 0.42, P=0.78 for blood pressure and blood gas analysis, respectively）. The brain temperature was significantly reduced in the group C compared to the other groups （F（2.45）=37.074, P=0.000; F（2.45）=32.983, P=0.000, for cortex and striatum temperature respectively）, while the difference in rectal temperature between group A and B or C after reperfusion was not significant （F（2.45）= 0.17115,P=0.637）. And the brain infarct volume was significantly reduced in group C （from 40%±10% in group A, 26%±8% in group B, to 12%±6% in group C, F（2.45）=43.465, P=0.000） with the neurological deficits improving in group C （Х^2=27.626, P=0.000）. The survival rate at 48 hours after 10℃ and 20℃ saline reperfusion was increased by 132.5% and 150%, respectively, as compared to the control group （Х^2=10.489, P=0.005）. The extenBackground Local hypothermia induced by intravascular infusion of cold saline solution effectively reduces brain damage in stroke. We further determined the optimal temperature of local hypothermia in our study. Methods Seventy-eight adult male Sprague Dawley rats （260-300 g） were randomly divided into 3 groups： group A, ischemia/reperfusion without cold saline infusion （n=26） （control group）; group B, infusion with 20℃ saline before reperfusion （n=26）; group C： infusion with 10℃ saline before reperfusion （n=26）. In each group, we chose 15 rats for monitoring physical indexes and the temperature of the brain （cortex and striatum） and body （anus）, measurement of brain infarction volume, assessment of neurological deficits and the survival rate of reperfusion at 48 hours. Another 8 rats from each group was chosen for examining brain edema, another 3 from each group for histological observation by electron microscopy （EM） and light microscopy （LM） at 48 hours after reperfusion. Results There was no significant difference among the 3 groups for physical indexes during the examination （F（2. 45）= 0.577, P=0.568; F（2.45）= 0.42, P=0.78 for blood pressure and blood gas analysis, respectively）. The brain temperature was significantly reduced in the group C compared to the other groups （F（2.45）=37.074, P=0.000; F（2.45）=32.983, P=0.000, for cortex and striatum temperature respectively）, while the difference in rectal temperature between group A and B or C after reperfusion was not significant （F（2.45）= 0.17115,P=0.637）. And the brain infarct volume was significantly reduced in group C （from 40%±10% in group A, 26%±8% in group B, to 12%±6% in group C, F（2.45）=43.465, P=0.000） with the neurological deficits improving in group C （Х^2=27.626, P=0.000）. The survival rate at 48 hours after 10℃ and 20℃ saline reperfusion was increased by 132.5% and 150%, respectively, as compared to the control group （Х^2=10.489, P=0.005）. The exten

关 键 词：RAT middle cerebral artery occlusion intra-arterial hyperthermia ischemia/reperfusion injury 

分 类 号：R651.15[医药卫生—外科学] R255.205[医药卫生—临床医学]